Issue 18, 2021

External stimuli-responsive nanoparticles for spatially and temporally controlled delivery of CRISPR–Cas genome editors

Abstract

The CRISPR–Cas9 system is a powerful tool for genome editing, which can potentially lead to new therapies for genetic diseases. To date, various viral and non-viral delivery systems have been developed for the delivery of CRISPR–Cas9 in vivo. However, spatially and temporally controlled genome editing is needed to enhance the specificity in organs/tissues and minimize the off-target effects of editing. In this review, we summarize the state-of-the-art non-viral vectors that exploit external stimuli (i.e., light, magnetic field, and ultrasound) for spatially and temporally controlled genome editing and their in vitro and in vivo applications.

Graphical abstract: External stimuli-responsive nanoparticles for spatially and temporally controlled delivery of CRISPR–Cas genome editors

Article information

Article type
Review Article
Submitted
07 Apr. 2021
Accepted
10 Jūn. 2021
First published
29 Jūn. 2021

Biomater. Sci., 2021,9, 6012-6022

External stimuli-responsive nanoparticles for spatially and temporally controlled delivery of CRISPR–Cas genome editors

R. Xie, Y. Wang and S. Gong, Biomater. Sci., 2021, 9, 6012 DOI: 10.1039/D1BM00558H

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