Issue 13, 2021

Simultaneous immunodetection of ionophore antibiotics, salinomycin and narasin, in poultry products and milk

Abstract

Rabbit polyclonal antibodies were generated against the ionophore antibiotic salinomycin (SAL) as a determinant of the BSA–SAL conjugate. The homologous ELISA format was found to be preferred for similar recognition of SAL and narasin (NAR) with IC50 values of 0.55 and 0.57 ng mL−1, respectively. Both analytes could be determined in the range of 0.1–2.7 ng mL−1 (IC20–IC80) with a detection limit of 0.03 ng mL−1. To analyze matrices, individual pretreatment of samples was required. For chicken muscles, simple buffer extraction was sufficient to recover 87–110% of ionophores. Extraction with acetonitrile followed by evaporation of the solvent was best for recovering 67–108% SAL and NAR from egg homogenate. A feature of the extraction of ionophores from milk was the elimination of fat-mediated interference by organic solvation. It was found that the absence of Na+ and K+ ions during reconstitution of sample extracts was a key factor contributing to the increase in the average recovery of ionophores from 32% to 93%. Thanks to this special pretreatment and improved recovery, the developed immunoassay method was suitable for the analysis of ionophore antibiotics SAL and NAR in a milk matrix, which has not been previously reported.

Graphical abstract: Simultaneous immunodetection of ionophore antibiotics, salinomycin and narasin, in poultry products and milk

Article information

Article type
Paper
Submitted
20 Dec. 2020
Accepted
03 Marts 2021
First published
19 Marts 2021

Anal. Methods, 2021,13, 1550-1558

Simultaneous immunodetection of ionophore antibiotics, salinomycin and narasin, in poultry products and milk

M. A. Burkin and I. A. Galvidis, Anal. Methods, 2021, 13, 1550 DOI: 10.1039/D0AY02309D

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