Issue 39, 2020

Consecutive dephosphorylation by alkaline phosphatase-directed in situ formation of porous hydrogels of SF with nanocrystalline calcium phosphate ceramics for bone regeneration

Abstract

Alkaline phosphatase, as an enzyme involved in the process of bone mineralization and regeneration, was incorporated into a solution of SF to induce its gelation and mineralization through consecutive dephosphorylation actions on different substrates. In these processes, alkaline phosphatase firstly worked on a small peptide of NapGFFYp by removing its hydrophilic phosphate group. The resulted NapGFFY performed supramolecular assembly in the solution of SF and synergistically induced the conformation transition of SF from random coil to β-sheet structures, leading to the formation of a stable SF hydrogel under physiological conditions. And then, the entrapped ALP within the SF–NY gel network retained its catalytic activity, released phosphate ions from glycerophosphate, and catalysed the formation of calcium phosphate minerals within the porous gel. Because of the mild conditions of these processes and good biocompatibility of the scaffold, the mineralized SF gel can work as a biomimetic scaffold to promote the osteogenic differentiation of rBMSCs and stimulate femoral defect regeneration in a rat model.

Graphical abstract: Consecutive dephosphorylation by alkaline phosphatase-directed in situ formation of porous hydrogels of SF with nanocrystalline calcium phosphate ceramics for bone regeneration

Supplementary files

Article information

Article type
Paper
Submitted
21 Jūl. 2020
Accepted
28 Aug. 2020
First published
31 Aug. 2020

J. Mater. Chem. B, 2020,8, 9043-9051

Consecutive dephosphorylation by alkaline phosphatase-directed in situ formation of porous hydrogels of SF with nanocrystalline calcium phosphate ceramics for bone regeneration

H. Li, J. Zhang, S. Liu, Y. Yan and X. Li, J. Mater. Chem. B, 2020, 8, 9043 DOI: 10.1039/D0TB01777A

To request permission to reproduce material from this article, please go to the Copyright Clearance Center request page.

If you are an author contributing to an RSC publication, you do not need to request permission provided correct acknowledgement is given.

If you are the author of this article, you do not need to request permission to reproduce figures and diagrams provided correct acknowledgement is given. If you want to reproduce the whole article in a third-party publication (excluding your thesis/dissertation for which permission is not required) please go to the Copyright Clearance Center request page.

Read more about how to correctly acknowledge RSC content.

Social activity

Spotlight

Advertisements