Issue 46, 2020

FRACTAL: Signal amplification of immunofluorescence via cyclic staining of target molecules

Abstract

In this article, we demonstrate fluorescent signal amplification via cyclic staining of target molecules (FRACTAL), a technique that can amplify the signal intensity of immunofluorescence staining more than nine-fold via simple cyclic staining of secondary antibodies. We also show that FRACTAL is compatible with four-color imaging and expansion microscopy imaging.

Graphical abstract: FRACTAL: Signal amplification of immunofluorescence via cyclic staining of target molecules

Supplementary files

Article information

Article type
Communication
Submitted
06 Aug. 2020
Accepted
28 Okt. 2020
First published
13 Nov. 2020

Nanoscale, 2020,12, 23506-23513

FRACTAL: Signal amplification of immunofluorescence via cyclic staining of target molecules

Y. Cho, J. Seo, Y. Sim, J. Chung, C. E. Park, C. G. Park, D. Kim and J. Chang, Nanoscale, 2020, 12, 23506 DOI: 10.1039/D0NR05800A

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