Issue 39, 2020

Controlling the non-linear emission of upconversion nanoparticles to enhance super-resolution imaging performance

Abstract

Upconversion nanoparticles (UCNPs) exhibit unique optical properties such as photo-emission stability, large anti-Stokes shift, and long excited-state lifetimes, allowing significant advances in a broad range of applications from biomedical sensing to super-resolution microscopy. In recent years, progress on nanoparticle synthesis led to the development of many strategies for enhancing their upconversion luminescence, focused in particular on heavy doping of lanthanide ions and core–shell structures. In this article, we investigate the non-linear emission properties of fully Yb-based core–shell UCNPs and their impact on the super-resolution performance of stimulated excitation-depletion (STED) microscopy and super-linear excitation-emission (uSEE) microscopy. Controlling the power-dependent emission curve enables us to relax constraints on the doping concentrations and to reduce the excitation power required for accessing sub-diffraction regimes. We take advantage of this feature to implement multiplexed super-resolution imaging of a two-sample mixture.

Graphical abstract: Controlling the non-linear emission of upconversion nanoparticles to enhance super-resolution imaging performance

Supplementary files

Article information

Article type
Paper
Submitted
26 Jūn. 2020
Accepted
24 Sept. 2020
First published
28 Sept. 2020
This article is Open Access
Creative Commons BY license

Nanoscale, 2020,12, 20347-20355

Controlling the non-linear emission of upconversion nanoparticles to enhance super-resolution imaging performance

S. De Camillis, P. Ren, Y. Cao, M. Plöschner, D. Denkova, X. Zheng, Y. Lu and J. A. Piper, Nanoscale, 2020, 12, 20347 DOI: 10.1039/D0NR04809G

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