Issue 2, 2020

The detection and identification of dengue virus serotypes with quantum dot and AuNP regulated localized surface plasmon resonance

Abstract

The dengue hemorrhagic fever or dengue shock syndrome has become a severe human fatal disease caused by infection with one of the four closely related but serologically distinct dengue viruses (DENVs). All four dengue serotypes are currently co-circulating throughout the subtropics and tropics. Since the fatality rate increases severely when a secondary infection occurs by a virus serotype different from that of the initial infection, serotype identification is equally important as virus detection. In this study, the development and validation of a rapid and quantitative DENV serotype-specific (serotypes 1–4) biosensor are reported by optimizing the stable system between cadmium selenide tellurium sulphide fluorescent quantum dots (CdSeTeS QDs) and gold nanoparticles (AuNPs). Four different nanoprobes are designed using each primer–probe serotype-specific hairpin single-stranded DNA covalently bound at different positions to CdSeTeS QDs, which generates an altered fluorescence signal for each serotype of DENV. In fourplex reactions with free functionalized AuNPs and the four nanoprobes, the standard dilutions of the target virus DNA from 10−15 to 10−10 M were successfully detected. The limit of detection was found to be in the femtomolar range for all four serotypes, where the serotype detection ability was undoubtedly established. To confirm the applicability of this sensing performance in long chained complex RNAs, the sensor was also applied successfully to RNAs extracted from DENV culture fluids for serotype identification as well as quantification, which can lead to a potential diagnostic probe for point-of-care detection.

Graphical abstract: The detection and identification of dengue virus serotypes with quantum dot and AuNP regulated localized surface plasmon resonance

Supplementary files

Article information

Article type
Paper
Submitted
04 Okt. 2019
Accepted
12 Dec. 2019
First published
13 Dec. 2019
This article is Open Access
Creative Commons BY-NC license

Nanoscale Adv., 2020,2, 699-709

The detection and identification of dengue virus serotypes with quantum dot and AuNP regulated localized surface plasmon resonance

A. D. Chowdhury, K. Takemura, I. M. Khorish, F. Nasrin, M. M. Ngwe Tun, K. Morita and E. Y. Park, Nanoscale Adv., 2020, 2, 699 DOI: 10.1039/C9NA00763F

This article is licensed under a Creative Commons Attribution-NonCommercial 3.0 Unported Licence. You can use material from this article in other publications, without requesting further permission from the RSC, provided that the correct acknowledgement is given and it is not used for commercial purposes.

To request permission to reproduce material from this article in a commercial publication, please go to the Copyright Clearance Center request page.

If you are an author contributing to an RSC publication, you do not need to request permission provided correct acknowledgement is given.

If you are the author of this article, you do not need to request permission to reproduce figures and diagrams provided correct acknowledgement is given. If you want to reproduce the whole article in a third-party commercial publication (excluding your thesis/dissertation for which permission is not required) please go to the Copyright Clearance Center request page.

Read more about how to correctly acknowledge RSC content.

Social activity

Spotlight

Advertisements