Issue 7, 2020

Rapid quantification of prion proteins using resistive pulse sensing

Abstract

Prion diseases are a group of fatal transmissible neurological conditions caused by the change in conformation of intrinsic cellular prion protein (PrPC). We present a rapid assay using aptamers and resistive pulse sensing, RPS, to extract and quantify PrPC from complex sample matrices. We functionalise the surface of superparamagnetic beads, SPBs, with a DNA aptamer. First SPB's termed P-beads, are used to pre-concentrate the analyte from a large sample volume. The PrPC protein is then eluted from the P-beads before aptamer modified sensing beads, S-beads, are added. The velocity of the S-beads through the nanopore reveals the concentration of the PrPC protein. The process is done in under an hour and allows the detection of picomol's of protein.

Graphical abstract: Rapid quantification of prion proteins using resistive pulse sensing

Supplementary files

Article information

Article type
Paper
Submitted
10 Janv. 2020
Accepted
11 Febr. 2020
First published
12 Febr. 2020

Analyst, 2020,145, 2595-2601

Rapid quantification of prion proteins using resistive pulse sensing

M. J. Healey, M. Sivakumaran and M. Platt, Analyst, 2020, 145, 2595 DOI: 10.1039/D0AN00063A

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