Issue 43, 2019

Two-dimensional UV spectroscopy: a new insight into the structure and dynamics of biomolecules

Abstract

Two-dimensional (2D) spectroscopy, originally developed for nuclear magnetic resonance, has been recently extended to the infrared and visible regimes. In this technique sequences of femtosecond light pulses are used to interrogate molecular systems and show, by a double Fourier transform, the correlation between excitation and detection frequencies. Extension to the ultraviolet (UV) regime is of great interest and promises to deliver rich structural and dynamical information on biomolecules such as DNA and proteins; however, it must overcome significant technical challenges. This review summarizes the current development status of 2DUV spectroscopy. After discussing the scientific case for the technique, we introduce its basic principles and review its experimental implementations, as well as the computational tools that have been developed to model the experiments. We conclude by giving a few application examples, which highlight the potential of 2DUV spectroscopy and motivate its further development.

Graphical abstract: Two-dimensional UV spectroscopy: a new insight into the structure and dynamics of biomolecules

Article information

Article type
Perspective
Submitted
03 Aug. 2019
Accepted
30 Sept. 2019
First published
15 Okt. 2019
This article is Open Access

All publication charges for this article have been paid for by the Royal Society of Chemistry
Creative Commons BY license

Chem. Sci., 2019,10, 9907-9921

Two-dimensional UV spectroscopy: a new insight into the structure and dynamics of biomolecules

R. Borrego-Varillas, A. Nenov, L. Ganzer, A. Oriana, C. Manzoni, A. Tolomelli, I. Rivalta, S. Mukamel, M. Garavelli and G. Cerullo, Chem. Sci., 2019, 10, 9907 DOI: 10.1039/C9SC03871J

This article is licensed under a Creative Commons Attribution 3.0 Unported Licence. You can use material from this article in other publications without requesting further permissions from the RSC, provided that the correct acknowledgement is given.

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