Issue 7, 2019

Posttranscriptional spin labeling of RNA by tetrazine-based cycloaddition

Abstract

The site-specific introduction of spin labels into RNA for distance measurements by EPR gives insight into its solution structure. We here present a method for spin labeling of in vitro transcribed RNA. Distance distributions between two nitroxide spin labels are determined by PELDOR in a self-complementary RNA duplex.

Graphical abstract: Posttranscriptional spin labeling of RNA by tetrazine-based cycloaddition

Supplementary files

Article information

Article type
Communication
Submitted
19 Okt. 2018
Accepted
27 Nov. 2018
First published
28 Nov. 2018

Org. Biomol. Chem., 2019,17, 1805-1808

Posttranscriptional spin labeling of RNA by tetrazine-based cycloaddition

C. Domnick, G. Hagelueken, F. Eggert, O. Schiemann and S. Kath-Schorr, Org. Biomol. Chem., 2019, 17, 1805 DOI: 10.1039/C8OB02597E

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