Issue 4, 2019

Graphene oxide prevents mycobacteria entry into macrophages through extracellular entrapment

Abstract

Tuberculosis (TB) remains a global threat and there is an urgent need for improved drugs and treatments, particularly against the drug-resistant strains of Mycobacterium tuberculosis (Mtb). Graphene oxide (GO) is an innovative bi-dimensional nanomaterial that when administered in vivo accumulates in the lungs. Further, GO is readily degraded by peroxidases and has a high drug loading capacity and antibacterial properties. In this study, we first evaluated the GO anti-mycobacterial properties using Mycobacterium smegmatis (Ms) as a model. We observed that GO, when administered with the bacteria, was able to trap Ms in a dose-dependent manner, reducing entry of bacilli into macrophages. However, GO did not show any anti-mycobacterial activity when used to treat infected cells or when macrophages were pre-treated before infection. Similar results were obtained when the virulent Mtb strain was used, showing that GO was able to trap Mtb and prevent entry into microphages. These results indicate that GO can be a promising tool to design improved therapies against TB.

Graphical abstract: Graphene oxide prevents mycobacteria entry into macrophages through extracellular entrapment

Article information

Article type
Paper
Submitted
22 Dec. 2018
Accepted
14 Janv. 2019
First published
16 Janv. 2019
This article is Open Access
Creative Commons BY-NC license

Nanoscale Adv., 2019,1, 1421-1431

Graphene oxide prevents mycobacteria entry into macrophages through extracellular entrapment

F. De Maio, V. Palmieri, A. Salustri, G. Perini, M. Sanguinetti, M. De Spirito, G. Delogu and M. Papi, Nanoscale Adv., 2019, 1, 1421 DOI: 10.1039/C8NA00413G

This article is licensed under a Creative Commons Attribution-NonCommercial 3.0 Unported Licence. You can use material from this article in other publications, without requesting further permission from the RSC, provided that the correct acknowledgement is given and it is not used for commercial purposes.

To request permission to reproduce material from this article in a commercial publication, please go to the Copyright Clearance Center request page.

If you are an author contributing to an RSC publication, you do not need to request permission provided correct acknowledgement is given.

If you are the author of this article, you do not need to request permission to reproduce figures and diagrams provided correct acknowledgement is given. If you want to reproduce the whole article in a third-party commercial publication (excluding your thesis/dissertation for which permission is not required) please go to the Copyright Clearance Center request page.

Read more about how to correctly acknowledge RSC content.

Social activity

Spotlight

Advertisements