Jump to main content
Jump to site search

Issue 10, 2018
Previous Article Next Article

How zinc ions shift and enhance the nucleotide's fluorescence spectra

Author affiliations

Abstract

The intrinsic fluorescence of four DNA nucleotides is particularly weak and their optical emission spectra are mutually almost indistinguishable. Therefore, intrinsic fluorescence was never considered as a physical property that could be utilized for optical detection and differentiation of nucleobases. In this article, we show how complexation of DNA nucleotides with zinc cations could change this issue: it strongly enhances the nucleotide fluorescence and shifts the fluorescence spectrum maxima by different amounts for different nucleotides. A detailed study of fluorescence in Zn(II) complexes with individual nucleotides – 2′-deoxyadenosine 5′-monophosphate (dAMP), thymidine 5′-monophosphate (TMP), 2′-deoxyguanosine 5′-monophosphate (dGMP) and 2′-deoxycytidine 5′-monophosphate (dCMP) – has shown that the strongest fluorescence amplification undergoes dGMP for which the integrated fluorescence intensity increases by two orders of magnitude upon complexing with Zn(II). The fluorescence spectra of the Zn(II)–nucleotide complexes are resolved well enough to enable discrimination of the nucleotides according to their optical emission spectra.

Graphical abstract: How zinc ions shift and enhance the nucleotide's fluorescence spectra

Back to tab navigation

Supplementary files

Article information


Submitted
27 Nov. 2017
Accepted
25 Febr. 2018
First published
01 Marts 2018

New J. Chem., 2018,42, 8145-8150
Article type
Paper

How zinc ions shift and enhance the nucleotide's fluorescence spectra

A. Omerzu and I. Turel, New J. Chem., 2018, 42, 8145 DOI: 10.1039/C7NJ04630H

To request permission to reproduce material from this article, please go to the Copyright Clearance Center request page.

If you are an author contributing to an RSC publication, you do not need to request permission provided correct acknowledgement is given.

If you are the author of this article, you do not need to request permission to reproduce figures and diagrams provided correct acknowledgement is given. If you want to reproduce the whole article in a third-party publication (excluding your thesis/dissertation for which permission is not required) please go to the Copyright Clearance Center request page.

Read more about how to correctly acknowledge RSC content.


Social activity

Search articles by author

Spotlight

Advertisements