Issue 11, 2018

The affinity of MhuD for heme is consistent with a heme degrading function in vivo

Abstract

MhuD is a protein found in mycobacteria that can bind up to two heme molecules per protein monomer and catalyze the degradation of heme to mycobilin in vitro. Here the Kd1 for heme dissociation from heme-bound MhuD was determined to be 7.6 ± 0.8 nM and the Kd2 for heme dissocation from diheme-bound MhuD was determined to be 3.3 ± 1.1 μM. These data strongly suggest that MhuD is a competent heme oxygenase in vivo.

Graphical abstract: The affinity of MhuD for heme is consistent with a heme degrading function in vivo

Supplementary files

Article information

Article type
Communication
Submitted
13 Aug. 2018
Accepted
13 Sept. 2018
First published
21 Sept. 2018

Metallomics, 2018,10, 1560-1563

Author version available

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