Issue 31, 2018

Impact of the lanthanide contraction on the activity of a lanthanide-dependent methanol dehydrogenase – a kinetic and DFT study

Abstract

Interest in the bioinorganic chemistry of lanthanides is growing rapidly as more and more lanthanide-dependent bacteria are being discovered. Especially the earlier lanthanides have been shown to be preferentially utilized by bacteria that need these Lewis acids as cofactors in their alcohol dehydrogenase enzymes. Here, we investigate the impact of the lanthanide ions lanthanum(III) to lutetium(III) (excluding Pm) on the catalytic parameters (vmax, KM, kcat/KM) of a methanol dehydrogenase (MDH) isolated from Methylacidiphilum fumariolicum SolV. Kinetic experiments and DFT calculations were used to discuss why only the earlier lanthanides (La–Gd) promote high MDH activity. Impact of Lewis acidity, coordination number preferences, stability constants and other properties that are a direct result of the lanthanide contraction are discussed in light of the two proposed mechanisms for MDH.

Graphical abstract: Impact of the lanthanide contraction on the activity of a lanthanide-dependent methanol dehydrogenase – a kinetic and DFT study

Supplementary files

Article information

Article type
Paper
Submitted
29 Marts 2018
Accepted
14 Jūn. 2018
First published
18 Jūl. 2018
This article is Open Access
Creative Commons BY-NC license

Dalton Trans., 2018,47, 10463-10472

Impact of the lanthanide contraction on the activity of a lanthanide-dependent methanol dehydrogenase – a kinetic and DFT study

H. Lumpe, A. Pol, H. J. M. Op den Camp and L. J. Daumann, Dalton Trans., 2018, 47, 10463 DOI: 10.1039/C8DT01238E

This article is licensed under a Creative Commons Attribution-NonCommercial 3.0 Unported Licence. You can use material from this article in other publications, without requesting further permission from the RSC, provided that the correct acknowledgement is given and it is not used for commercial purposes.

To request permission to reproduce material from this article in a commercial publication, please go to the Copyright Clearance Center request page.

If you are an author contributing to an RSC publication, you do not need to request permission provided correct acknowledgement is given.

If you are the author of this article, you do not need to request permission to reproduce figures and diagrams provided correct acknowledgement is given. If you want to reproduce the whole article in a third-party commercial publication (excluding your thesis/dissertation for which permission is not required) please go to the Copyright Clearance Center request page.

Read more about how to correctly acknowledge RSC content.

Social activity

Spotlight

Advertisements