Issue 20, 2018

A ratiometric Raman probe for live-cell imaging of hydrogen sulfide in mitochondria by stimulated Raman scattering

Abstract

Stimulated Raman Scattering (SRS) coupled with alkyne tags has been an emerging imaging technique to visualize small-molecule species with high sensitivity and specificity. Here we describe the development of a ratiometric Raman probe for visualizing hydrogen sulfide (H2S) species in living cells as the first alkyne-based sensor for SRS microscopy. This probe uses an azide unit as a selective reactive site, and it targets mitochondria with high specificity. The SRS ratiometric images show a strong response to H2S level changes in living cells.

Graphical abstract: A ratiometric Raman probe for live-cell imaging of hydrogen sulfide in mitochondria by stimulated Raman scattering

Supplementary files

Article information

Article type
Communication
Submitted
16 Maijs 2018
Accepted
31 Aug. 2018
First published
03 Sept. 2018

Analyst, 2018,143, 4844-4848

A ratiometric Raman probe for live-cell imaging of hydrogen sulfide in mitochondria by stimulated Raman scattering

C. Zeng, F. Hu, R. Long and W. Min, Analyst, 2018, 143, 4844 DOI: 10.1039/C8AN00910D

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