Issue 32, 2017

Onion derived carbon nanodots for live cell imaging and accelerated skin wound healing

Abstract

Nitrogen, sulfur, and phosphorous co-doped water-soluble carbon nanodots are synthesized from culinary waste onion peel powder (OPP) by a short microwave treatment. Onion Derived Carbon Nano Dots (OCND) that comprised hydrophilic group-decorated amorphous nano-dots exhibited bright, stable fluorescence at an excitation of 450 nm and emission wavelength at 520 nm along with a free radical scavenging property. The OCND exhibited excellent stability at different pH and UV exposure. Although extracted polyphenols degraded in the extract, interestingly it was shown to be cytocompatible and blood compatible as observed during cytotoxicity, fluorescence imaging of the cell and a hemolysis study. The present work not only focuses on the synthesis of OCND from the OPP extract but also provides an interesting fact that, even after the degradation of polyphenols in the extract, they are non-toxic to human cells (HFF & MG63) and RBCs. Moreover, OCND had no adverse effect on the migration rate of Human Foreskin-derived Fibroblasts (HFFs) as observed from a scratch assay. In addition to accelerating the migration rate of fibroblasts, the OCND altered intra- and extracellular reactive oxygen species (ROS) by enhancing the antioxidant mechanism of a fibroblast under oxidative stress. Further, OCND was observed to accelerate wound healing in a full thickness (FT) wound in a rat model for topical application, which can be attributed to its radical scavenging potential. In summary, this study leads to a new type of OCND synthesis route, which is inherently co-doped with phosphorous, sulfur and nitrogen and holds a great promise for a myriad of biological applications, including bio-imaging, free radical scavenging and wound healing.

Graphical abstract: Onion derived carbon nanodots for live cell imaging and accelerated skin wound healing

Article information

Article type
Paper
Submitted
30 Marts 2017
Accepted
01 Jūn. 2017
First published
01 Jūn. 2017

J. Mater. Chem. B, 2017,5, 6579-6592

Onion derived carbon nanodots for live cell imaging and accelerated skin wound healing

K. Bankoti, A. P. Rameshbabu, S. Datta, B. Das, A. Mitra and S. Dhara, J. Mater. Chem. B, 2017, 5, 6579 DOI: 10.1039/C7TB00869D

To request permission to reproduce material from this article, please go to the Copyright Clearance Center request page.

If you are an author contributing to an RSC publication, you do not need to request permission provided correct acknowledgement is given.

If you are the author of this article, you do not need to request permission to reproduce figures and diagrams provided correct acknowledgement is given. If you want to reproduce the whole article in a third-party publication (excluding your thesis/dissertation for which permission is not required) please go to the Copyright Clearance Center request page.

Read more about how to correctly acknowledge RSC content.

Social activity

Spotlight

Advertisements