Issue 32, 2017

Functionalization of bone implants with nanodiamond particles and angiopoietin-1 to improve vascularization and bone regeneration

Abstract

One of the major challenges in bone tissue engineering is adequate vascularization within bone substituents for nutrients and oxygen supply. In this study, the production and results of a new, highly functional bone construct consisting of a commercial three-dimensional β-tricalcium phosphate scaffold (β-TCP, ChronOS®) and hydrophilic, functionalized nanodiamond (ND) particles are reported. A 30-fold increase in the active surface area of the ChronOS + ND scaffold was achieved after modification with ND. In addition, immobilization of angiopoietin-1 (Ang-1) via physisorption within the β-TCP + ND scaffold retained the bioactivity of the growth factor. Homogeneous distribution of the ND and Ang-1 within the core of the three-dimensional scaffold was confirmed using ND covalently labelled with Oregon Green. The biological responses of the β-TCP + ND scaffold with and without Ang-1 were studied in a sheep calvaria critical size defect model showing that the β-TCP + ND scaffold improved the blood vessel ingrowth and the β-TCP + ND + ND + Ang-1 scaffold further promoted vascularization and new bone formation. The results demonstrate that the modification of scaffolds with tailored diamond nanoparticles is a valuable method for improving the characteristics of bone implants and enables new approaches in bone tissue engineering.

Graphical abstract: Functionalization of bone implants with nanodiamond particles and angiopoietin-1 to improve vascularization and bone regeneration

Supplementary files

Article information

Article type
Paper
Submitted
15 Marts 2017
Accepted
31 Maijs 2017
First published
31 Maijs 2017

J. Mater. Chem. B, 2017,5, 6629-6636

Functionalization of bone implants with nanodiamond particles and angiopoietin-1 to improve vascularization and bone regeneration

X. Wu, M. Bruschi, T. Waag, S. Schweeberg, Y. Tian, T. Meinhardt, R. Stigler, K. Larsson, M. Funk, D. Steinmüller-Nethl, M. Rasse and A. Krueger, J. Mater. Chem. B, 2017, 5, 6629 DOI: 10.1039/C7TB00723J

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