Issue 2, 2017

A mitochondria-targeted ratiometric fluorescent probe for hypochlorite and its applications in bioimaging

Abstract

A novel ratiometric probe (RCP) for OCl was developed based on the fluorescence resonance energy transfer (FRET) platform. The probe was constructed by integrating the coumarin moiety (FRET donor) with the rhodamine moiety (FRET acceptor). Upon treatment with OCl, the coumarin emission at 483 nm decreased and the rhodamine emission at 570 nm increased, enabling the probe to provide accurate detection of OCl (in the concentration range of 0–50 μM). The probe exhibited brilliant selectivity and sensitivity, rapid response and low cytotoxicity. More importantly, the introduction of the quaternized pyridine moiety can not only manage to increase the solubility, but also achieve mitochondria-targeting. The probe was applied successfully to imaging endogenous OCl in mitochondria, highlighting its potential applications in bioanalysis.

Graphical abstract: A mitochondria-targeted ratiometric fluorescent probe for hypochlorite and its applications in bioimaging

Supplementary files

Article information

Article type
Paper
Submitted
08 Aug. 2016
Accepted
23 Nov. 2016
First published
23 Nov. 2016

J. Mater. Chem. B, 2017,5, 289-295

A mitochondria-targeted ratiometric fluorescent probe for hypochlorite and its applications in bioimaging

S. Shen, X. Zhao, X. Zhang, X. Liu, H. Wang, Y. Dai, J. Miao and B. Zhao, J. Mater. Chem. B, 2017, 5, 289 DOI: 10.1039/C6TB01992G

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