Issue 2, 2017

Reversible ratiometric detection of highly reactive hydropersulfides using a FRET-based dual emission fluorescent probe

Abstract

Hydropersulfide (R–SSH) is an important class of reactive sulfur species (RSS) involved in a variety of physiological processes in mammals. A fluorescent probe capable of real-time detection of hydropersulfide levels in living cells would be a versatile tool to elucidate its roles in cell signalling and redox homeostasis. In this paper, we report a ratiometric fluorescent probe for hydropersulfide sensing, based on a fluorescence resonance energy transfer (FRET) mechanism. This sensing mechanism involves a nucleophilic reaction of a hydropersulfide with the pyronine-unit of the probe, which modulates the intramolecular FRET efficiency to induce a dual-emission change. The reversible nature of this reaction allows us to detect increases and decreases of hydropersulfide levels in a real-time manner. The probe fluorometrically sensed highly reactive hydropersulfides, such as H2S2 and Cys-SSH, while the fluorescence response to biologically abundant cysteine and glutathione was negligible. Taking advantage of the reversible and selective sensing properties, this probe was successfully applied to the ratiometric imaging of concentration dynamics of endogenously produced hydropersulfides in living cells.

Graphical abstract: Reversible ratiometric detection of highly reactive hydropersulfides using a FRET-based dual emission fluorescent probe

Supplementary files

Article information

Article type
Edge Article
Submitted
29 Aug. 2016
Accepted
23 Sept. 2016
First published
26 Sept. 2016
This article is Open Access

All publication charges for this article have been paid for by the Royal Society of Chemistry
Creative Commons BY license

Chem. Sci., 2017,8, 1134-1140

Reversible ratiometric detection of highly reactive hydropersulfides using a FRET-based dual emission fluorescent probe

R. Kawagoe, I. Takashima, S. Uchinomiya and A. Ojida, Chem. Sci., 2017, 8, 1134 DOI: 10.1039/C6SC03856E

This article is licensed under a Creative Commons Attribution 3.0 Unported Licence. You can use material from this article in other publications without requesting further permissions from the RSC, provided that the correct acknowledgement is given.

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