Issue 13, 2017

Pre-concentration by liquid intake by paper (P-CLIP): a new technique for large volumes and digital microfluidics

Abstract

Microfluidic platforms are an attractive option for incorporating complex fluid handling into low-cost and rapid diagnostic tests. A persistent challenge for microfluidics, however, is the mismatch in the “world-to-chip” interface – it is challenging to detect analytes present at low concentrations in systems that can only handle small volumes of sample. Here we describe a new technique termed pre-concentration by liquid intake by paper (P-CLIP) that addresses this mismatch, allowing digital microfluidics to interface with volumes on the order of hundreds of microliters. In P-CLIP, a virtual microchannel is generated to pass a large volume through the device; analytes captured on magnetic particles can be isolated and then resuspended into smaller volumes for further processing and analysis. We characterize this method and demonstrate its utility with an immunoassay for Plasmodium falciparum lactate dehydrogenase, a malaria biomarker, and propose that the P-CLIP strategy may be useful for a wide range of applications that are currently limited by low-abundance analytes.

Graphical abstract: Pre-concentration by liquid intake by paper (P-CLIP): a new technique for large volumes and digital microfluidics

Supplementary files

Article information

Article type
Paper
Submitted
23 Apr. 2017
Accepted
02 Jūn. 2017
First published
12 Jūn. 2017
This article is Open Access
Creative Commons BY license

Lab Chip, 2017,17, 2272-2280

Pre-concentration by liquid intake by paper (P-CLIP): a new technique for large volumes and digital microfluidics

D. G. Rackus, R. P. S. de Campos, C. Chan, M. M. Karcz, B. Seale, T. Narahari, C. Dixon, M. D. Chamberlain and A. R. Wheeler, Lab Chip, 2017, 17, 2272 DOI: 10.1039/C7LC00440K

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