Issue 53, 2017

Incorporation of native antibodies and Fc-fusion proteins on DNA nanostructures via a modular conjugation strategy

Abstract

A photocrosslinkable protein G variant was used as an adapter protein to covalently and site-specifically conjugate an antibody and an Fc-fusion protein to an oligonucleotide. This modular approach enables straightforward decoration of DNA nanostructures with complex native proteins while retaining their innate binding affinity, allowing precise control over the nanoscale spatial organization of such proteins for in vitro and in vivo biomedical applications.

Graphical abstract: Incorporation of native antibodies and Fc-fusion proteins on DNA nanostructures via a modular conjugation strategy

Supplementary files

Article information

Article type
Communication
Submitted
30 Maijs 2017
Accepted
09 Jūn. 2017
First published
09 Jūn. 2017
This article is Open Access
Creative Commons BY license

Chem. Commun., 2017,53, 7393-7396

Incorporation of native antibodies and Fc-fusion proteins on DNA nanostructures via a modular conjugation strategy

B. J. H. M. Rosier, G. A. O. Cremers, W. Engelen, M. Merkx, L. Brunsveld and T. F. A. de Greef, Chem. Commun., 2017, 53, 7393 DOI: 10.1039/C7CC04178K

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