Issue 53, 2017

Live-cell monochromatic dual-label sub-diffraction microscopy by mt-pcSOFI

Abstract

We expand photochromic super-resolution optical fluctuation imaging (pcSOFI) to monochromatic dual-channel sub-diffraction microscopy. Multi-tau (mt-)pcSOFI unmixes spectrally identical reversibly switchable fluorescent proteins (RSFPs) based on their blinking kinetics. We show that mt-pcSOFI can be used to simultaneously image two structures in living cells with existing RSFPs and the newly developed ffDronpa-F.

Graphical abstract: Live-cell monochromatic dual-label sub-diffraction microscopy by mt-pcSOFI

Supplementary files

Article information

Article type
Communication
Submitted
27 Marts 2017
Accepted
19 Maijs 2017
First published
31 Maijs 2017

Chem. Commun., 2017,53, 7242-7245

Live-cell monochromatic dual-label sub-diffraction microscopy by mt-pcSOFI

S. Duwé, W. Vandenberg and P. Dedecker, Chem. Commun., 2017, 53, 7242 DOI: 10.1039/C7CC02344H

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