Issue 19, 2017

Nucleic acid detection using BRET-beacons based on bioluminescent protein–DNA hybrids

Abstract

Bioluminescent molecular beacons have been developed using a modular design approach that relies on BRET between the bright luciferase NanoLuc and a Cy3 acceptor. While classical molecular beacons are hampered by background fluorescence and scattering, these BRET-beacons allow detection of low pM concentrations of nucleic acids directly in complex media.

Graphical abstract: Nucleic acid detection using BRET-beacons based on bioluminescent protein–DNA hybrids

Supplementary files

Article information

Article type
Communication
Submitted
16 Dec. 2016
Accepted
15 Febr. 2017
First published
15 Febr. 2017
This article is Open Access
Creative Commons BY-NC license

Chem. Commun., 2017,53, 2862-2865

Nucleic acid detection using BRET-beacons based on bioluminescent protein–DNA hybrids

W. Engelen, K. M. van de Wiel, L. H. H. Meijer, B. Saha and M. Merkx, Chem. Commun., 2017, 53, 2862 DOI: 10.1039/C6CC10032E

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