Issue 20, 2016

Pluripotent stem cell derived hepatocytes: using materials to define cellular differentiation and tissue engineering

Abstract

Pluripotent stem cell derived liver cells (hepatocytes) represent a promising alternative to primary tissue for biological and clinical applications. To date, most hepatocyte maintenance and differentiation systems have relied upon the use of animal derived components. This serves as a significant barrier to large scale production and application of stem cell derived hepatocytes. Recently, the use of defined biologics has overcome those limitations in two-dimensional monolayer culture. In order to improve the cell phenotype further, three-dimensional culture systems have been employed to better mimic the in vivo situation, drawing upon materials chemistry, engineering and biology. In this review we discuss efforts in the field, to differentiate pluripotent stem cells towards hepatocytes under defined conditions.

Graphical abstract: Pluripotent stem cell derived hepatocytes: using materials to define cellular differentiation and tissue engineering

Article information

Article type
Review Article
Submitted
05 Febr. 2016
Accepted
14 Apr. 2016
First published
15 Apr. 2016
This article is Open Access
Creative Commons BY license

J. Mater. Chem. B, 2016,4, 3433-3442

Pluripotent stem cell derived hepatocytes: using materials to define cellular differentiation and tissue engineering

B. Lucendo-Villarin, H. Rashidi, K. Cameron and D. C. Hay, J. Mater. Chem. B, 2016, 4, 3433 DOI: 10.1039/C6TB00331A

This article is licensed under a Creative Commons Attribution 3.0 Unported Licence. You can use material from this article in other publications without requesting further permissions from the RSC, provided that the correct acknowledgement is given.

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