Issue 3, 2016

Time-resolved proteomic analysis of quorum sensing in Vibrio harveyi

Abstract

Bacteria use a process of chemical communication called quorum sensing to assess their population density and to change their behavior in response to fluctuations in the cell number and species composition of the community. In this work, we identified the quorum-sensing-regulated proteome in the model organism Vibrio harveyi by bio-orthogonal non-canonical amino acid tagging (BONCAT). BONCAT enables measurement of proteome dynamics with temporal resolution on the order of minutes. We deployed BONCAT to characterize the time-dependent transition of V. harveyi from individual- to group-behaviors. We identified 176 quorum-sensing-regulated proteins at early, intermediate, and late stages of the transition, and we mapped the temporal changes in quorum-sensing proteins controlled by both transcriptional and post-transcriptional mechanisms. Analysis of the identified proteins revealed 86 known and 90 new quorum-sensing-regulated proteins with diverse functions, including transcription factors, chemotaxis proteins, transport proteins, and proteins involved in iron homeostasis.

Graphical abstract: Time-resolved proteomic analysis of quorum sensing in Vibrio harveyi

Supplementary files

Article information

Article type
Edge Article
Submitted
04 Sept. 2015
Accepted
17 Nov. 2015
First published
23 Nov. 2015
This article is Open Access

All publication charges for this article have been paid for by the Royal Society of Chemistry
Creative Commons BY license

Chem. Sci., 2016,7, 1797-1806

Author version available

Time-resolved proteomic analysis of quorum sensing in Vibrio harveyi

J. D. Bagert, J. C. van Kessel, M. J. Sweredoski, L. Feng, S. Hess, B. L. Bassler and D. A. Tirrell, Chem. Sci., 2016, 7, 1797 DOI: 10.1039/C5SC03340C

This article is licensed under a Creative Commons Attribution 3.0 Unported Licence. You can use material from this article in other publications without requesting further permissions from the RSC, provided that the correct acknowledgement is given.

Read more about how to correctly acknowledge RSC content.

Social activity

Spotlight

Advertisements