Issue 9, 2016

A gold nanoparticle-based semi-quantitative and quantitative ultrasensitive paper sensor for the detection of twenty mycotoxins

Abstract

A semi-quantitative and quantitative multi-immunochromatographic (ICA) strip detection assay was developed for the simultaneous detection of twenty types of mycotoxins from five classes, including zearalenones (ZEAs), deoxynivalenols (DONs), T-2 toxins (T-2s), aflatoxins (AFs), and fumonisins (FBs), in cereal food samples. Sensitive and specific monoclonal antibodies were selected for this assay. The semi-quantitative results were obtained within 20 min by the naked eye, with visual limits of detection for ZEAs, DONs, T-2s, AFs and FBs of 0.1–0.5, 2.5–250, 0.5–1, 0.25–1 and 2.5–10 μg kg−1, and cut-off values of 0.25–1, 5–500, 1–10, 0.5–2.5 and 5–25 μg kg−1, respectively. The quantitative results were obtained using a hand-held strip scan reader, with the calculated limits of detection for ZEAs, DONs, T-2s, AFs and FBs of 0.04–0.17, 0.06–49, 0.15–0.22, 0.056–0.49 and 0.53–1.05 μg kg−1, respectively. The analytical results of spiked samples were in accordance with the accurate content in the simultaneous detection analysis. This newly developed ICA strip assay is suitable for the on-site detection and rapid initial screening of mycotoxins in cereal samples, facilitating both semi-quantitative and quantitative determination.

Graphical abstract: A gold nanoparticle-based semi-quantitative and quantitative ultrasensitive paper sensor for the detection of twenty mycotoxins

Supplementary files

Article information

Article type
Paper
Submitted
24 Dec. 2015
Accepted
04 Febr. 2016
First published
05 Febr. 2016

Nanoscale, 2016,8, 5245-5253

A gold nanoparticle-based semi-quantitative and quantitative ultrasensitive paper sensor for the detection of twenty mycotoxins

D. Kong, L. Liu, S. Song, S. Suryoprabowo, A. Li, H. Kuang, L. Wang and C. Xu, Nanoscale, 2016, 8, 5245 DOI: 10.1039/C5NR09171C

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