Issue 6, 2016

Design and development of histone deacetylase (HDAC) chemical probes for cell-based profiling

Abstract

Histone deacetylases (HDACs) contribute to regulation of gene expression by mediating higher-order chromatin structures. They assemble into large multiprotein complexes that regulate activity and specificity. We report the development of small molecule probes with class IIa and pan-HDAC activity that contain photoreactive crosslinking groups and either a biotin reporter, or a terminal alkyne handle for subsequent bioorthogonal ligation. The probes retained inhibitory activity against recombinant HDAC proteins and caused an accumulation of acetylated histone and tubulin following cell treatment. The versatility of the probes has been demonstrated by their ability to photoaffinity modify HDAC targets in vitro. An affinity enrichment probe was used in conjunction with mass spectrometry proteomics to isolate HDACs and their interacting proteins in a native proteome. The performance of the probes in recombinant versus cell-based systems highlights issues for the development of chemoproteomic technologies targeting class IIa HDACs in particular.

Graphical abstract: Design and development of histone deacetylase (HDAC) chemical probes for cell-based profiling

Supplementary files

Article information

Article type
Paper
Submitted
10 Febr. 2016
Accepted
21 Marts 2016
First published
29 Marts 2016

Mol. BioSyst., 2016,12, 1781-1789

Design and development of histone deacetylase (HDAC) chemical probes for cell-based profiling

V. E. Albrow, R. L. Grimley, J. Clulow, C. R. Rose, J. Sun, J. S. Warmus, E. W. Tate, L. H. Jones and R. I. Storer, Mol. BioSyst., 2016, 12, 1781 DOI: 10.1039/C6MB00109B

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