Issue 3, 2016

Handheld real-time PCR device

Abstract

Here we report one of the smallest real-time polymerase chain reaction (PCR) systems to date with an approximate size of 100 mm × 60 mm × 33 mm. The system is an autonomous unit requiring an external 12 V power supply. Four simultaneous reactions are performed in the form of virtual reaction chambers (VRCs) where a ≈200 nL sample is covered with mineral oil and placed on a glass cover slip. Fast, 40 cycle amplification of an amplicon from the H7N9 gene was used to demonstrate the PCR performance. The standard curve slope was −3.02 ± 0.16 cycles at threshold per decade (mean ± standard deviation) corresponding to an amplification efficiency of 0.91 ± 0.05 per cycle (mean ± standard deviation). The PCR device was capable of detecting a single deoxyribonucleic acid (DNA) copy. These results further suggest that our handheld PCR device may have broad, technologically-relevant applications extending to rapid detection of infectious diseases in small clinics.

Graphical abstract: Handheld real-time PCR device

Supplementary files

Article information

Article type
Paper
Submitted
18 Nov. 2015
Accepted
16 Dec. 2015
First published
16 Dec. 2015
This article is Open Access
Creative Commons BY license

Lab Chip, 2016,16, 586-592

Handheld real-time PCR device

C. D. Ahrberg, B. R. Ilic, A. Manz and P. Neužil, Lab Chip, 2016, 16, 586 DOI: 10.1039/C5LC01415H

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