Issue 35, 2016

Dengue diagnosis on laser printed microzones using smartphone-based detection and multivariate image analysis

Abstract

This study describes the use of smartphone-based detection and multivariate image analysis (MIA) to perform dengue diagnosis on laser printed microzones. Toner-based microplates containing 96 microzones were fabricated on a transparency film surface through a single laser printing step. Enzyme-linked-immunosorbent assay (ELISA) experiments were then carried out to detect both immunoglobulin M (IgM) and G (IgG) antibodies in serum samples collected from patients infected and not infected with dengue virus. Principal component analysis (PCA) of ELISA digital image data acquired with a smartphone revealed good discrimination between samples from infected and non-infected patients.

Graphical abstract: Dengue diagnosis on laser printed microzones using smartphone-based detection and multivariate image analysis

Supplementary files

Article information

Article type
Paper
Submitted
28 Apr. 2016
Accepted
04 Aug. 2016
First published
05 Aug. 2016

Anal. Methods, 2016,8, 6506-6511

Dengue diagnosis on laser printed microzones using smartphone-based detection and multivariate image analysis

K. A. Oliveira, D. Damasceno, C. R. de Oliveira, L. A. da Silveira, A. E. de Oliveira and W. K. T. Coltro, Anal. Methods, 2016, 8, 6506 DOI: 10.1039/C6AY01262K

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