Issue 12, 2016

Ex vivo expansion of circulating lung tumor cells based on one-step microfluidics-based immunomagnetic isolation

Abstract

We describe a one-step microfludics-based immunomagnetic isolation method to isolate CTCs directly from the whole blood of lung adenocarcinoma patients. This method avoids harsh sample preparation and enrichment steps, and therefore preserves the viability of CTCs during the in vitro isolation. Importantly, isolated, magnetic bead-bearing CTCs are concentrated in a small volume of culture medium with a high CTC density. High cell viability and culturing density promote the ex vivo expansion of limited numbers of CTCs. Expanded CTCs are characterized at the genetic, protein and metabolic levels.

Graphical abstract: Ex vivo expansion of circulating lung tumor cells based on one-step microfluidics-based immunomagnetic isolation

Supplementary files

Article information

Article type
Communication
Submitted
12 Dec. 2015
Accepted
09 Febr. 2016
First published
09 Febr. 2016

Analyst, 2016,141, 3621-3625

Ex vivo expansion of circulating lung tumor cells based on one-step microfluidics-based immunomagnetic isolation

Z. Wang, W. Wu, Z. Wang, Y. Tang, Y. Deng, L. Xu, J. Tian and Q. Shi, Analyst, 2016, 141, 3621 DOI: 10.1039/C5AN02554K

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