Issue 3, 2015

Photostick: a method for selective isolation of target cells from culture

Abstract

Sorting of target cells from a heterogeneous pool is technically difficult when the selection criterion is complex, e.g. a dynamic response, a morphological feature, or a combination of multiple parameters. At present, mammalian cell selections are typically performed either via static fluorescence (e.g. fluorescence activated cell sorter), via survival (e.g. antibiotic resistance), or via serial operations (flow cytometry, laser capture microdissection). Here we present a simple protocol for selecting cells based on any static or dynamic property that can be identified by video microscopy and image processing. The “photostick” technique uses a cell-impermeant photochemical crosslinker and digital micromirror array-based patterned illumination to immobilize selected cells on the culture dish. Other cells are washed away with mild protease treatment. The crosslinker also labels the selected cells with a fluorescent dye and a biotin for later identification. The photostick protocol preserves cell viability, permits genetic profiling of selected cells, and can be performed with complex functional selection criteria such as neuronal firing patterns.

Graphical abstract: Photostick: a method for selective isolation of target cells from culture

Supplementary files

Article information

Article type
Edge Article
Submitted
27 Nov. 2014
Accepted
07 Janv. 2015
First published
21 Janv. 2015
This article is Open Access

All publication charges for this article have been paid for by the Royal Society of Chemistry
Creative Commons BY-NC license

Chem. Sci., 2015,6, 1701-1705

Author version available

Photostick: a method for selective isolation of target cells from culture

M. Chien, C. A. Werley, S. L. Farhi and A. E. Cohen, Chem. Sci., 2015, 6, 1701 DOI: 10.1039/C4SC03676J

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