Issue 14, 2015

Quantum dots: bright and versatile in vitro and in vivo fluorescence imaging biosensors

Abstract

Semiconductor quantum dots (QDs) have become important fluorescent probes for in vitro and in vivo bioimaging research. Their nanoparticle surfaces for versatile bioconjugation, their adaptable photophysical properties for multiplexed detection, and their superior stability for longer investigation times are the main advantages of QDs compared to other fluorescence imaging agents. Here, we review the recent literature dealing with the design and application of QD-bioconjugates for advanced in vitro and in vivo imaging. After a short summary of QD preparation and their most important properties, different QD-based imaging applications will be discussed from the technological and the biological point of view, ranging from super-resolution microscopy and single-particle tracking over in vitro cell and tissue imaging to in vivo investigations. A substantial part of the review will focus on multifunctional applications, in which the QD fluorescence is combined with drug or gene delivery towards theranostic approaches or with complementary technologies for multimodal imaging. We also briefly discuss QD toxicity issues and give a short outlook on future directions of QD-based bioimaging.

Graphical abstract: Quantum dots: bright and versatile in vitro and in vivo fluorescence imaging biosensors

Article information

Article type
Review Article
Submitted
31 Dec. 2014
First published
17 Marts 2015
This article is Open Access
Creative Commons BY-NC license

Chem. Soc. Rev., 2015,44, 4792-4834

Quantum dots: bright and versatile in vitro and in vivo fluorescence imaging biosensors

K. D. Wegner and N. Hildebrandt, Chem. Soc. Rev., 2015, 44, 4792 DOI: 10.1039/C4CS00532E

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