Issue 39, 2015

Biosynthetic selenoproteins with genetically-encoded photocaged selenocysteines

Abstract

Selenocysteine is a valuable component of both natural selenoproteins and designer biocatalysts; however the availability of such proteins is hampered by technical limitations. Here we report the first general strategy for the production of selenoproteins via genetically-encoded incorporation of a synthetic photocaged selenocysteine residue in yeast cells, and provide examples of light-controlled protein dimerization and targeted covalent labeling in vitro.

Graphical abstract: Biosynthetic selenoproteins with genetically-encoded photocaged selenocysteines

Supplementary files

Article information

Article type
Communication
Submitted
07 Okt. 2014
Accepted
05 Febr. 2015
First published
05 Febr. 2015
This article is Open Access
Creative Commons BY-NC license

Chem. Commun., 2015,51, 8245-8248

Biosynthetic selenoproteins with genetically-encoded photocaged selenocysteines

R. Rakauskaitė, G. Urbanavičiūtė, A. Rukšėnaitė, Z. Liutkevičiūtė, R. Juškėnas, V. Masevičius and S. Klimašauskas, Chem. Commun., 2015, 51, 8245 DOI: 10.1039/C4CC07910H

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