Issue 7, 2015

Comparison of transmission and transflectance mode FTIR imaging of biological tissue

Abstract

FTIR microscopy is a powerful technique which has become popular due to its ability to provide complementary information during histopathological assessment of biomedical tissue samples. Recently however, questions have been raised on the suitability of the transflection mode of operation for clinical diagnosis due to the so called Electric Field Standing Wave (EFSW) effect. In this paper we compare chemical images measured in transmission and transflection from prostate tissue obtained from five different patients, and discuss the variability of the spectra acquired with each sampling modality. We find that spectra obtained in transflection undergo a non-linear distortion, i.e. non-linear variations in absorption band strength across the spectra, and that there are significant differences in spectra measured from the same area of tissue depending on the mode of operation. Principal Component Analysis (PCA) is used to highlight that poorer discrimination between benign and cancerous tissue is obtained in transflection mode. In addition we show that use of second derivatives, while qualitatively improves spectral discrimination, does not completely alleviate the underlying problem.

Graphical abstract: Comparison of transmission and transflectance mode FTIR imaging of biological tissue

Article information

Article type
Paper
Submitted
29 Okt. 2014
Accepted
02 Febr. 2015
First published
02 Febr. 2015

Analyst, 2015,140, 2383-2392

Author version available

Comparison of transmission and transflectance mode FTIR imaging of biological tissue

M. J. Pilling, P. Bassan and P. Gardner, Analyst, 2015, 140, 2383 DOI: 10.1039/C4AN01975J

To request permission to reproduce material from this article, please go to the Copyright Clearance Center request page.

If you are an author contributing to an RSC publication, you do not need to request permission provided correct acknowledgement is given.

If you are the author of this article, you do not need to request permission to reproduce figures and diagrams provided correct acknowledgement is given. If you want to reproduce the whole article in a third-party publication (excluding your thesis/dissertation for which permission is not required) please go to the Copyright Clearance Center request page.

Read more about how to correctly acknowledge RSC content.

Social activity

Spotlight

Advertisements