Issue 39, 2013

Artificial repressors for controlling gene expression in bacteria

Abstract

Transcriptional repression is a common approach to control gene expression in synthetic biology applications. Here, an engineered DNA binding protein based upon a transcription activator-like effector (TALE) scaffold was shown to outperform LacI in blocking transcription from a promoter and to repress expression of a downstream gene in an operon.

Graphical abstract: Artificial repressors for controlling gene expression in bacteria

Supplementary files

Article information

Article type
Communication
Submitted
29 Sept. 2012
Accepted
16 Nov. 2012
First published
19 Nov. 2012

Chem. Commun., 2013,49, 4325-4327

Artificial repressors for controlling gene expression in bacteria

M. C. Politz, M. F. Copeland and B. F. Pfleger, Chem. Commun., 2013, 49, 4325 DOI: 10.1039/C2CC37107C

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