Issue 7, 2024

Intracellular microbial rhodopsin-based optogenetics to control metabolism and cell signaling

Abstract

Microbial rhodopsin (MRs) ion channels and pumps have become invaluable optogenetic tools for neuroscience as well as biomedical applications. Recently, MR-optogenetics expanded towards subcellular organelles opening principally new opportunities in optogenetic control of intracellular metabolism and signaling via precise manipulations of organelle ion gradients using light. This new optogenetic field expands the opportunities for basic and medical studies of cancer, cardiovascular, and metabolic disorders, providing more detailed and accurate control of cell physiology. This review summarizes recent advances in studies of the cellular metabolic processes and signaling mediated by optogenetic tools targeting mitochondria, endoplasmic reticulum (ER), lysosomes, and synaptic vesicles. Finally, we discuss perspectives of such an optogenetic approach in both fundamental and applied research.

Graphical abstract: Intracellular microbial rhodopsin-based optogenetics to control metabolism and cell signaling

Supplementary files

Article information

Article type
Review Article
Submitted
27 Aug. 2023
First published
23 Febr. 2024

Chem. Soc. Rev., 2024,53, 3327-3349

Intracellular microbial rhodopsin-based optogenetics to control metabolism and cell signaling

A. D. Vlasova, S. M. Bukhalovich, D. F. Bagaeva, A. P. Polyakova, N. S. Ilyinsky, S. V. Nesterov, F. M. Tsybrov, A. O. Bogorodskiy, E. V. Zinovev, A. E. Mikhailov, A. V. Vlasov, A. I. Kuklin, V. I. Borshchevskiy, E. Bamberg, V. N. Uversky and V. I. Gordeliy, Chem. Soc. Rev., 2024, 53, 3327 DOI: 10.1039/D3CS00699A

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