Issue 15, 2024

A chemiluminescence immunoassay for type IV collagen as a promising marker for liver fibrosis and cirrhosis

Abstract

Herein, a magnetic bead-based chemiluminescence assay is reported to detect type IV collagen (col-IV) in serum samples. Magnetic beads (MBs) exhibit biocompatibility. Taking advantage of this property, they were conjugated with the col-IV antibody. For the determination of col-IV, the interaction of the col-IV sample, anti-(col-IV)-alkaline phosphatase (anti-(col-IV)-ALP) and anti-col-IV-magnetic beads (anti-(col-IV)-MBs) was performed to generate chemiluminescence. Under the optimized conditions, the developed method displayed good linearity in the concentration range of 20–2000 ng mL−1 with the limit of 0.79 ng mL−1. The repeatability coefficient of variation (CV) for col-IV detection ranged from 3.16% to 7.50%. The col-IV level in samples collected from a hospital was assessed by the chemiluminescence assay. Satisfactory recoveries were obtained ranging from 93.30% to 100.14%. In conclusion, the magnetic bead-based chemiluminescence assay may be used as a routine and efficient tool to detect type IV collagen in clinical diagnosis.

Graphical abstract: A chemiluminescence immunoassay for type IV collagen as a promising marker for liver fibrosis and cirrhosis

Article information

Article type
Paper
Submitted
15 Dec. 2023
Accepted
27 Febr. 2024
First published
26 Marts 2024

Anal. Methods, 2024,16, 2248-2255

A chemiluminescence immunoassay for type IV collagen as a promising marker for liver fibrosis and cirrhosis

X. Fu, F. Zhang, F. Zhen, L. Duan, J. Zhou and J. Ma, Anal. Methods, 2024, 16, 2248 DOI: 10.1039/D3AY02240D

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