Issue 18, 2022

Simple manipulation of enzyme-linked immunosorbent assay (ELISA) using an automated microfluidic interface

Abstract

Among lateral flow immunoassay (LFIA) platforms, enzyme-based LFIAs provide signal amplification to improve sensitivity. However, most enzyme-based LFIAs require multiple timed steps, complicating their utility in point-of-care testing (POCT). Here, we report a microfluidic interface for LFIAs that automates sample, buffer, and reagent addition, greatly simplifying operation while achieving the high analytical stringency associated with more complex assays. The microfluidic interface also maintains the low cost and small footprint of standard LFIAs. The platform is fabricated from a combination of polyester film, double-sided adhesive tape, and nitrocellulose, and fits in the palm of your hand. All reagents are dried on the nitrocellulose to facilitate sequential reagent delivery, and the sample is used as the wash buffer to minimize steps. After the sample addition, a user simply waits 15 min for a colorimetric result. This manuscript discusses the development and optimization of the channel geometry to achieve a simple step enzyme amplified immunoassay. As a proof-of-concept target, we selected lipoarabinomannan (LAM), a WHO identified urinary biomarker of active tuberculosis, to demonstrate the device feasibility and reliability. The results revealed that the device successfully detected LAM in phosphate buffer (PBS) as well as spiked urine samples within 15 min after sample loading. The minimum concentration of color change was achieved at 25 ng mL−1.

Graphical abstract: Simple manipulation of enzyme-linked immunosorbent assay (ELISA) using an automated microfluidic interface

Supplementary files

Article information

Article type
Paper
Submitted
25 Febr. 2022
Accepted
08 Apr. 2022
First published
21 Apr. 2022

Anal. Methods, 2022,14, 1774-1781

Simple manipulation of enzyme-linked immunosorbent assay (ELISA) using an automated microfluidic interface

Y. Panraksa, I. Jang, C. S. Carrell, A. G. Amin, O. Chailapakul, D. Chatterjee and C. S. Henry, Anal. Methods, 2022, 14, 1774 DOI: 10.1039/D2AY00326K

To request permission to reproduce material from this article, please go to the Copyright Clearance Center request page.

If you are an author contributing to an RSC publication, you do not need to request permission provided correct acknowledgement is given.

If you are the author of this article, you do not need to request permission to reproduce figures and diagrams provided correct acknowledgement is given. If you want to reproduce the whole article in a third-party publication (excluding your thesis/dissertation for which permission is not required) please go to the Copyright Clearance Center request page.

Read more about how to correctly acknowledge RSC content.

Social activity

Spotlight

Advertisements