Issue 99, 2021

Pyridinium-conjugated polynorbornenes for nanomolar ATP sensing using an indicator displacement assay and a PET strategy

Abstract

An indicator displacement assay, namely polymeric PNPY-n/UD consisting of a cationic polynorbornene backbone with pyridinium functional groups (PNPY-1,2,3) and an anionic uranine dye (UD) as an indicator, has been developed for highly sensitive “turn-on” fluorescence sensing of ATP. While PNPY-1/UD itself is non-emissive, a bright green fluorescence signal was observed in the presence of ATP [Ka = 2.17 × 105 M−1, LOD = 5.7 nM]. The potential of a highly photostable system PNPY-1/UD was also validated in detecting ATP levels in live-cell imaging applications.

Graphical abstract: Pyridinium-conjugated polynorbornenes for nanomolar ATP sensing using an indicator displacement assay and a PET strategy

Supplementary files

Article information

Article type
Communication
Submitted
29 Sept. 2021
Accepted
23 Nov. 2021
First published
23 Nov. 2021

Chem. Commun., 2021,57, 13530-13533

Pyridinium-conjugated polynorbornenes for nanomolar ATP sensing using an indicator displacement assay and a PET strategy

L. Huang, Y. He, J. Kim, A. Sharma, Q. Cao and J. S. Kim, Chem. Commun., 2021, 57, 13530 DOI: 10.1039/D1CC05500C

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