Issue 19, 2021

Application of centrifugal microfluidics in immunoassay, biochemical analysis and molecular diagnosis

Abstract

Rapid diagnosis plays a vital role in daily life and is effective in reducing treatment costs and increasing curability, especially in remote areas with limited availability of resources. Among the various common methods of rapid diagnosis, centrifugal microfluidics has many unique advantages, such as less sample consumption, more precise valve control for sequential loading of samples, and accurately separated module design in a microfluidic network to minimize cross-contamination. Therefore, in recent years, centrifugal microfluidics has been extensively researched, and it has been found to play important roles in biology, chemistry, and medicine. Here, we review the latest developments in centrifugal microfluidic platforms in immunoassays, biochemical analyses, and molecular diagnosis, in recent years. In immunoassays, we focus on the application of enzyme-linked immunosorbent assay (ELISA); in biochemical analysis, we introduce the application of plasma and blood cell separation; and in molecular diagnosis, we highlight the application of nucleic acid amplification tests. Additionally, we discuss the characteristics of the methods under each platform as well as the enhancement of the corresponding performance parameters, such as the limit of detection, separation efficiency, etc. Finally, we discuss the limitations associated with the existing applications and potential breakthroughs that can be achieved in this field in the future.

Graphical abstract: Application of centrifugal microfluidics in immunoassay, biochemical analysis and molecular diagnosis

Article information

Article type
Critical Review
Submitted
13 Apr. 2021
Accepted
20 Jūl. 2021
First published
04 Aug. 2021

Analyst, 2021,146, 5800-5821

Application of centrifugal microfluidics in immunoassay, biochemical analysis and molecular diagnosis

Y. Shi, P. Ye, K. Yang, J. Meng, J. Guo, Z. Pan, W. Zhao and J. Guo, Analyst, 2021, 146, 5800 DOI: 10.1039/D1AN00629K

To request permission to reproduce material from this article, please go to the Copyright Clearance Center request page.

If you are an author contributing to an RSC publication, you do not need to request permission provided correct acknowledgement is given.

If you are the author of this article, you do not need to request permission to reproduce figures and diagrams provided correct acknowledgement is given. If you want to reproduce the whole article in a third-party publication (excluding your thesis/dissertation for which permission is not required) please go to the Copyright Clearance Center request page.

Read more about how to correctly acknowledge RSC content.

Social activity

Spotlight

Advertisements