Issue 13, 2019

Mechanistic characterization of three sesquiterpene synthases from the termite-associated fungus Termitomyces

Abstract

Three terpene synthases from the termite associated fungus Termitomyces were functionally characterized as (+)-intermedeol synthase, (−)-γ-cadinene synthase and (+)-germacrene D-4-ol synthase, with the germacrene D-4-ol synthase as the first reported enzyme that produces the (+)-enantiomer. The enzymatic mechanisms were thoroughly investigated by incubation with isotopically labeled precursors to follow the stereochemical courses of single reaction steps in catalysis. The role of putative active site residues was tested by site directed mutagenesis of a highly conserved tryptophan in all three enzymes and additional residues in (−)-γ-cadinene synthase that were identified by homology model analysis.

Graphical abstract: Mechanistic characterization of three sesquiterpene synthases from the termite-associated fungus Termitomyces

Supplementary files

Article information

Article type
Paper
Submitted
05 Nov. 2018
Accepted
22 Janv. 2019
First published
22 Janv. 2019

Org. Biomol. Chem., 2019,17, 3348-3355

Mechanistic characterization of three sesquiterpene synthases from the termite-associated fungus Termitomyces

I. Burkhardt, N. B. Kreuzenbeck, C. Beemelmanns and J. S. Dickschat, Org. Biomol. Chem., 2019, 17, 3348 DOI: 10.1039/C8OB02744G

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