Issue 15, 2019

A comparison of serum and plasma specimens in NMR-based metabonomics and its application in chronic atrophic gastritis rats

Abstract

The choice of blood specimens is still disputable in metabonomic studies due to the great metabolic diversities of serum and plasma. In the present work, a proton nuclear magnetic resonance spectroscopy (NMR)-based metabonomic approach was introduced to explore metabolic differences between serum and plasma coupled with its application in the pathogenesis of chronic atrophic gastritis (CAG). As a result, 3 and 20 identified metabolites were applied to characterize the metabolic differences between serum and plasma, respectively, in control or CAG rats, which might be related to the effect of anticoagulants. Almost all the metabolic contents except lactate and β-glucose in serum samples were higher than those in plasma samples. Meanwhile, 12 serum and 11 plasma potential biomarkers were detected to relate to the formation of CAG by comparisons of the normal and CAG groups, respectively. Receiver operating characteristic analysis (ROC) also showed that serum specimens were clearly superior to plasma based on sensitivity of the diagnosis of CAG. The results showed that the combination of them could enhance the metabolic coverage related to the mechanism of CAG, which involves oxidative stress, energy failure, cell injury and death. Furthermore, selecting a serum specimen might be a good way to investigate the blood metabolome, especially under pathological states. This may favor the use of serum rather than plasma in NMR-based metabonomics.

Graphical abstract: A comparison of serum and plasma specimens in NMR-based metabonomics and its application in chronic atrophic gastritis rats

Supplementary files

Article information

Article type
Paper
Submitted
21 Janv. 2019
Accepted
13 Marts 2019
First published
14 Marts 2019

Anal. Methods, 2019,11, 2018-2026

A comparison of serum and plasma specimens in NMR-based metabonomics and its application in chronic atrophic gastritis rats

Y. Liu, W. Xu, G. Wang, A. Li, K. Li and X. Qin, Anal. Methods, 2019, 11, 2018 DOI: 10.1039/C9AY00156E

To request permission to reproduce material from this article, please go to the Copyright Clearance Center request page.

If you are an author contributing to an RSC publication, you do not need to request permission provided correct acknowledgement is given.

If you are the author of this article, you do not need to request permission to reproduce figures and diagrams provided correct acknowledgement is given. If you want to reproduce the whole article in a third-party publication (excluding your thesis/dissertation for which permission is not required) please go to the Copyright Clearance Center request page.

Read more about how to correctly acknowledge RSC content.

Social activity

Spotlight

Advertisements