Issue 44, 2018

Conductive silk–polypyrrole composite scaffolds with bioinspired nanotopographic cues for cardiac tissue engineering

Abstract

We report on the development of bioinspired cardiac scaffolds made from electroconductive acid-modified silk fibroin–poly(pyrrole) (AMSF + PPy) substrates patterned with nanoscale ridges and grooves reminiscent of native myocardial extracellular matrix (ECM) topography to enhance the structural and functional properties of cultured human pluripotent stem cells (hPSC)-derived cardiomyocytes. Nanopattern fidelity was maintained throughout the fabrication and functionalization processes, and no loss in conductive behavior occurred due to the presence of the nanotopographical features. AMSF + PPy substrates were biocompatible and stable, maintaining high cell viability over a 21 day culture period while displaying no signs of PPy delamination. The presence of anisotropic topographical cues led to increased cellular organization and sarcomere development, and electroconductive cues promoted a significant improvement in the expression and polarization of connexin 43 (Cx43), a critical regulator of cell–cell electrical coupling. The combination of biomimetic topography and electroconductivity also increased the expression of genes that encode key proteins involved in regulating the contractile and electrophysiological function of mature human cardiac tissue.

Graphical abstract: Conductive silk–polypyrrole composite scaffolds with bioinspired nanotopographic cues for cardiac tissue engineering

Supplementary files

Article information

Article type
Paper
Submitted
27 Apr. 2018
Accepted
18 Jūn. 2018
First published
18 Jūn. 2018

J. Mater. Chem. B, 2018,6, 7185-7196

Author version available

Conductive silk–polypyrrole composite scaffolds with bioinspired nanotopographic cues for cardiac tissue engineering

J. H. Tsui, N. A. Ostrovsky-Snider, D. M. P. Yama, J. D. Donohue, J. S. Choi, R. Chavanachat, J. D. Larson, A. R. Murphy and D. Kim, J. Mater. Chem. B, 2018, 6, 7185 DOI: 10.1039/C8TB01116H

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