Issue 24, 2018

FRET and PET paired dual mechanistic carbon dots approach for tyrosinase sensing

Abstract

A dual mechanistic FRET and PET paired ratiometric fluorescence sensor probe has been prepared using carbon dots and naphthalimide fluorophores. The carbon dots are covalently joined with a naphthalimide moiety to develop the FRET phenomenon, which emits at two different wavelengths (i.e., λmax = 440 and 540 nm). However, on catalytic reaction of tyrosinase, the fluorescence emission intensity of the acceptor unit at 540 nm is quenched gradually, owing to the switching on of the PET mechanism; while emission of the donor unit remains significantly unaffected. The probe exhibits high selectivity and specificity towards tyrosinase in complex biological medium with a detection limit of 1.2 U mL−1. Moreover, endogenous images of tyrosinase in B16 cells have been observed under a confocal laser-scanning microscope.

Graphical abstract: FRET and PET paired dual mechanistic carbon dots approach for tyrosinase sensing

Supplementary files

Article information

Article type
Paper
Submitted
23 Febr. 2018
Accepted
18 Maijs 2018
First published
21 Maijs 2018

J. Mater. Chem. B, 2018,6, 4139-4145

FRET and PET paired dual mechanistic carbon dots approach for tyrosinase sensing

J. S. Sidhu and N. Singh, J. Mater. Chem. B, 2018, 6, 4139 DOI: 10.1039/C8TB00512E

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