Issue 24, 2010

Immuno-pillar chip: a new platform for rapid and easy-to-use immunoassay

Abstract

We present a new rapid and easy-to-use immunoassay chip which we have named the immuno-pillar chip. It has hydrogel pillars, fabricated inside a microchannel, with many antibody molecules immobilized onto 1 µm diameter polystyrene beads. To evaluate the chip performance, we applied it to the sandwich assay of C-reactive protein (CRP), α-fetoprotein (AFP) and prostate-specific antigen (PSA), a cardiac and inflammation marker, tumors and prostate cancer markers, respectively. For detection of disease markers, we confirmed the chip provides rapid analysis (total assay time of about 4 min) with high sensitivity, it is easy-to-use (no special skills are needed), and it uses small volumes of the sample and reagent (0.25 µL each). Moreover, multiplex assay for three biomarkers was also possible. Additionally, the immuno-pillar chip has a big advantage of having hardly any influence on the assay results even if the introduction quantities of the sample or reagents are different.

Graphical abstract: Immuno-pillar chip: a new platform for rapid and easy-to-use immunoassay

Supplementary files

Article information

Article type
Paper
Submitted
26 Jūl. 2010
Accepted
22 Sept. 2010
First published
19 Okt. 2010

Lab Chip, 2010,10, 3335-3340

Immuno-pillar chip: a new platform for rapid and easy-to-use immunoassay

M. Ikami, A. Kawakami, M. Kakuta, Y. Okamoto, N. Kaji, M. Tokeshi and Y. Baba, Lab Chip, 2010, 10, 3335 DOI: 10.1039/C0LC00241K

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