Themed collection Most popular 2022 analytical chemistry articles

This review presents a current account of hyperpolarized ¹⁵N-labeled molecular imaging probes, as well as insights on their advantages and challenges to advance future development of ¹⁵N-based probes and their applications in MRS/MRI.

Misfolded α-synuclein oligomers are closely implicated in the pathology of Parkinson's disease and related synucleinopathies.

A new chemiluminescence neuraminidase probe enables rapid screening of small molecules that inhibit viral replication, directly in influenza A-infected mammalian cells.

Small-molecule near-infrared (NIR) imaging facilitates deep tissue penetration, low autofluorescence, non-invasive visualization, and a relatively simple operation.

SECM using FcCOOH as the redox mediator and endogenous GSH as the indicator was employed to investigate the effect of extracellular matrix stiffness on the functional activity of MRP1 in cancer cells in situ.

O-PTIR was used for simultaneous collection of infrared and Raman spectra from clinical pathogens associated with bloodstream infections.

Ultraviolet photodissociation mass spectrometry is used to probe the paratopes of nanobodies, a class of single-domain antibodies, and to determine intersubunit salt-bridges and explore the nanobody·antigen interfaces.

An intelligent enantioselective identification strategy is designed to demonstrate that both enantioselectivity and stereoselectivity are crucial factors for chiral sensing.

A standard-free stable single magnetic bead nanoplatform was proposed in this work. The use of metal signal ratio was directly applied for intrinsic biological fPSA to tPSA ratio evaluations for prostate disease differentiation.

Functionalization of the bridging silicon atom of Si-rhodamine dyes allows tuning of dye performance, the attachment of sensors, and the addition of biomolecular targeting ligands useful for the construction of live cell imaging probes.

Detection and visualization of the cannabinoid receptor type 2 by a cell-permeable high affinity fluorescent probe platform enables tracing receptor trafficking in live cells and in zebrafish.

Oligonucleotide drug delivery approach is provided with a biomimetic, dynamic DNA nanomaterial, which enables disease gene regulation and auxiliary therapy in a controllable and self-boosting manner.

Inhibitory effects of paracetamol on the oxidase-like activity of Fe single-atom nanozymes.

A triple molecularly imprinted polymer (MIP)-based plasmonic assay was developed for precise cancer diagnosis in terms of the relative glycosylation expression of glycoprotein biomarkers.

A caged imidazopyrazinone can detect reactive extracellular copper in mammalian systems using bioluminescence generated with the ATP-independent nanoluciferase enzyme.

Herein, we demonstrate that MnO₂ nanosheets are an excellent carrier of CRISPR/Cas12a due to the two important roles played by them.

Developing a novel tool capable of real-time monitoring and accurate quantification of NO is critical to understanding its role in physiological and pathological processes.

A four-state kinetics model is proposed to reveal the kinetics of a single-molecule reaction under nanopore confinement.

Carbonylpyridinium and chromene were elaborately assembled to highly target mitochondrial thiol assay by releasing o-quinone methide from CM-Mit to label proteins, thus avoiding diffusion out of the mitochondria, which enabled accurately spatiotemporal detection of thiol.

¹⁹F-centred NMR structure determination protocol alleviates the need for compound separation. Disinfection byproducts of chloramination were unraveled by analyzing the reaction pathways of a single fluorinated molecule.

Stimulated Raman scattering microscopy with spectral phasor analysis provides a label-free approach for phenotypic evaluation of drug-induced effects.

A H₂S triggered and H₂S releasing near-infrared fluorescent probe (HL-H₂S) was developed. HL-H₂S does not interfere with the progression of ferroptosis by consuming H₂S, thus enabling high-fidelity in situ imaging of ferroptosis.

A novel bioconjugatable and photostable AIE luminogen has been rationally synthesized for precise and long-term tracking of neuron mitochondria.

A. Gallo Jr, H. Mishra et al., pinpoint the origins of the spontaneous H₂O₂ formation in water microdroplets formed via spraying or condensation, i.e., without the addition of electrical energy, catalyst, or co-solvent.

We report the first “total synthesis” of ¹⁷O-labeled D-glucose and its solid-state ¹⁷O NMR characterization with unprecedented sensitivity and resolution.

The first chemiluminescent probe for the detection of LAP is described. It shows a highly selective, sensitive and rapid chemiluminescence response for the detection of LAP in vitro and in vivo, and enables the differentiation of liver cancer.

This study illustrates that 2′-O-methyl modified gRNAs improve the specificity of the CRISPR–Cas12a system (mg-CRISPR) via suppressing the Cas12a's affinity to off-target DNA and provides an efficient strategy for high-specificity gRNA design.

This work developed the PAM-less conditional DNA substrates that leverage the trans-cleavage effect of CRISPR-Cas12a to sense various biomolecules in living cells.

Label-free mass cytometry is utilized to study the dynamic metabolic change during apoptosis in HepG2 cells induced by NK92 MI cells at the single-cell level. The metabolic heterogeneity of individual HepG2 cells during apoptosis was revealed.

An “OFF–ON–OFF” fluorescence probe for real-time imaging of the expression (fluorescence ‘OFF’) and degradation (fluorescence ‘ON’) of short lived PYP-tag proteins in cellular systems.