Point-of-care diagnostics: recent developments in a pandemic age

Harshit Harpaldas, Siddarth Arumugam, Chelsey Campillo Rodriguez, Bhoomika Ajay Kumar, Vivian Shi and Samuel K. Sia

Lab Chip, 2021, 21, 4517
DOI: 10.1039/D1LC00627D

Reagent free detection of SARS-CoV-2 using an antibody-based microwave sensor in a microfluidic platform

Weijia Cui, Pei Zhao, Jin Wang, Ning Qin, Emmanuel A. Ho and Carolyn L. Ren

Lab Chip, 2022, 22, 2307
DOI: 10.1039/D2LC00145D

Immuno-biosensor on a chip: a self-powered microfluidic-based electrochemical biosensing platform for point-of-care quantification of proteins

Fatemeh Haghayegh, Razieh Salahandish, Azam Zare, Mahmood Khalghollah and Amir Sanati-Nezhad

Lab Chip, 2022, 22, 108
DOI: 10.1039/D1LC00879J

Advances in isothermal nucleic acid amplification methods for hepatitis B virus detection

Huilin Li, Wenjun Song, Hongying Li, Jiaqi Cui, Yuchen Xie, Bo Wu and Rong Chen

Analyst, 2023, 148, 3708
DOI: 10.1039/D3AN00700F

Portable and simultaneous detection of four respiratory pathogens through a microfluidic LAMP and real-time fluorescence assay

Junwen Liu, Zhi Zeng, Feina Li, Bo Jiang, You Nie, Guohao Zhang, Biao Pang, Lin Sun and Rongzhang Hao

Analyst, 2024, 149, 5091
DOI: 10.1039/D4AN00748D

Diagnosis of disease relevant nucleic acid biomarkers with off-the-shelf devices

Lijuan Qi and Yan Du

J. Mater. Chem. B, 2022, 10, 3959
DOI: 10.1039/D2TB00232A

An integrated nucleic acid detection method based on a microfluidic chip for collection and culture of rice false smut spores

Ning Yang, Yuanyuan Ji, Aiying Wang, Jian Tang, Shuhua Liu, Xiaodong Zhang, Lijia Xu and Yong He

Lab Chip, 2022, 22, 4894
DOI: 10.1039/D2LC00931E

SARS-CoV-2 recombinase polymerase amplification assay with lateral flow readout and duplexed full process internal control

Coleman D. Martin, Andrew T. Bender, Benjamin P. Sullivan, Lorraine Lillis, David S. Boyle and Jonathan D. Posner

Sens. Diagn., 2024, 3, 421
DOI: 10.1039/D3SD00246B

Microfluidic methods for the diagnosis of acute respiratory tract infections

Kan-Zhi Liu, Ganghong Tian, Alex C.-T. Ko, Matthias Geissler, Lidija Malic, Byeong-Ui Moon, Liviu Clime and Teodor Veres

Analyst, 2025, 150, 9
DOI: 10.1039/D4AN00957F

Tetra-primer ARMS-PCR combined with dual-color fluorescent lateral flow assay for the discrimination of SARS-CoV-2 and its mutations with a handheld wireless reader

Yunxiang Wang, Hong Chen, Hongjuan Wei, Zhen Rong and Shengqi Wang

Lab Chip, 2022, 22, 1531
DOI: 10.1039/D1LC01167G

Smartphone clip-on instrument and microfluidic processor for rapid sample-to-answer detection of Zika virus in whole blood using spatial RT-LAMP

Aaron M. Jankelow, Hankeun Lee, Weijing Wang, Trung-Hieu Hoang, Amanda Bacon, Fu Sun, Seol Chae, Victoria Kindratenko, Katherine Koprowski, Robert A. Stavins, Dylann D. Ceriani, Zachary W. Engelder, William P. King, Minh N. Do, Rashid Bashir, Enrique Valera and Brian T. Cunningham

Analyst, 2022, 147, 3838
DOI: 10.1039/D2AN00438K

Enhanced portable detection for Sars-CoV-2 utilizing DNA tetrahedron-tethered aptamers and a pressure meter

Xingbo Yu, Ying Wang, Kun Wang, Ziyuan Zhu, Lu Xiao, Yishun Huang, Yanling Song and Dan Liu

Anal. Methods, 2024, 16, 639
DOI: 10.1039/D3AY02100A

Hydraulic–electric analogy for design and operation of microfluidic systems

Zhenglin Li, Chao Liu and Jiashu Sun

Lab Chip, 2023, 23, 3311
DOI: 10.1039/D3LC00265A

Development of pseudo 3D covalent organic framework nanosheets for sensitive and selective biomolecule detection of infectious disease

Nargish Parvin, Tapas K. Mandal and Sang W. Joo

J. Mater. Chem. C, 2023, 11, 16398
DOI: 10.1039/D3TC03231K

COVID-19 diagnostics: Molecular biology to nanomaterials

Summaya Perveen, Anjali Negi, Varun Gopalakrishnan, Satyajit Panda, Vinay Sharma and Rashmi Sharma

Clinica Chimica Acta, 2023, 538, 139
DOI: 10.1016/j.cca.2022.11.017

RT-RPA Assay Combined with a Lateral Flow Strip to Detect Soybean Mosaic Virus

Bong Geun Oh, Ju-Yeon Yoon and Ho-Jong Ju

Plant Pathol J, 2024, 40, 337
DOI: 10.5423/PPJ.OA.02.2024.0027

Microfluidic chip and isothermal amplification technologies for the detection of pathogenic nucleic acid

Dongli Gao, Xudong Guo, Yi Yang, Hua Shi, Rongzhang Hao, Shengqi Wang, Zhen Jun Li, Rongtao Zhao and Hongbin Song

J Biol Eng, 2022, 16
DOI: 10.1186/s13036-022-00312-w

Unlocking the Future: Carbon Nanotubes as Pioneers in Sensing Technologies

Nargish Parvin, Sang Woo Joo, Jae Hak Jung and Tapas K. Mandal

Chemosensors, 2025, 13, 225
DOI: 10.3390/chemosensors13070225

Droplet Microfluidics for High-Resolution Virology

Wenyang Jing and Hee-Sun Han

Anal. Chem., 2022, 94, 8085
DOI: 10.1021/acs.analchem.2c00615

SMART: A Swing-Assisted Multiplexed Analyzer for Point-of-Care Respiratory Tract Infection Testing

Li Zhang, Xu Wang, Dongchen Liu, Yu Wu, Li Feng, Chunyan Han, Jiajia Liu, Ying Lu, Dmitriy V. Sotnikov, Youchun Xu and Jing Cheng

Biosensors, 2023, 13, 228
DOI: 10.3390/bios13020228

Rapid and Sensitive Detection of Verticillium dahliae from Soil Using LAMP-CRISPR/Cas12a Technology

Yuxiao Fang, Lijuan Liu, Wenyuan Zhao, Linpeng Dong, Lijuan He, Yuhan Liu, Jinyao Yin, Yufang Zhang, Weiguo Miao and Daipeng Chen

IJMS, 2024, 25, 5185
DOI: 10.3390/ijms25105185

Gold Nanoparticle Enabled Localized Surface Plasmon Resonance on Unique Gold Nanomushroom Structures for On‐Chip CRISPR‐Cas13a Sensing

Jacob Waitkus, Yu Chang, Li Liu, Srinivasu Valagerahally Puttaswamy, Taerin Chung, Adrian M. Molina Vargas, Stephen J. Dollery, Mitchell R. O'Connell, Haogang Cai, Gregory J. Tobin, Nikhil Bhalla and Ke Du

Adv Materials Inter, 2023, 10
DOI: 10.1002/admi.202201261

Detection of HBV pgRNA by catalytic hairpin assembly combined with two Kinds of immunochromatographic strips

Yaya Chen, Yuming Yao, Shuo Ma, Meiling Zhou, Jiwei Wang, Gulinaizhaer Abudushalamu, Shijie Cai, Xun Gao, Xiaobo Fan and Guoqiu Wu

Microchemical Journal, 2024, 207, 112075
DOI: 10.1016/j.microc.2024.112075

Fully automated sample to result SIMPLE RPA microfluidic chip towards in ovo sexing application

Simão Monteiro Belo dos Santos, Celine Wegsteen, Dries Vloemans, Matthias Corion, Bart De Ketelaere, Dragana Spasic and Jeroen Lammertyn

npj Biosensing, 2024, 1
DOI: 10.1038/s44328-024-00019-6

Unifying the Efforts of Medicine, Chemistry, and Engineering in Biosensing Technologies to Tackle the Challenges of the COVID-19 Pandemic

Özgecan Erdem, Ismail Eş, Yeşeren Saylan and Fatih Inci

Anal. Chem., 2022, 94, 3
DOI: 10.1021/acs.analchem.1c04454

Multiple ligation–Assisted recombinase polymerase amplification for highly sensitive and selective colorimetric detection of SARS-CoV-2

Tasnima Alam Asa, Pradeep Kumar, Jaehyeon Lee and Young Jun Seo

Talanta, 2023, 252, 123835
DOI: 10.1016/j.talanta.2022.123835

Rapid, Highly Sensitive, and Label-Free Pathogen Assay System Using a Solid-Phase Self-Interference Recombinase Polymerase Amplification Chip and Hyperspectral Interferometry

Xiangyu Jin, Rongxin Fu, Wenli Du, Xiaohui Shan, Zeyin Mao, Anni Deng, Xue Lin, Ya Su, Han Yang, Wenqi Lv, Hao Zhong and Guoliang Huang

Anal. Chem., 2022, 94, 2926
DOI: 10.1021/acs.analchem.1c04858

In Situ Complexation of sgRNA and Cas12a Improves the Performance of a One-Pot RPA–CRISPR-Cas12 Assay

Jake M. Lesinski, Thomas Moragues, Prerit Mathur, Yang Shen, Carolina Paganini, Léonard Bezinge, Bo Verberckmoes, Bodine Van Eenooghe, Stavros Stavrakis, Andrew J. deMello and Daniel A. Richards

Anal. Chem., 2024, 96, 10443
DOI: 10.1021/acs.analchem.4c01777

A precise review on NAATs‐based diagnostic assays for COVID‐19: A motion in fast POC molecular tests

Bahareh Maleki and Zohreh Hojati

Eur J Clin Investigation, 2022, 52
DOI: 10.1111/eci.13853

Bimetallic Gold--Platinum (AuPt) Nanozymes: Recent Advances in Synthesis and Applications for Food Safety Monitoring

Shipeng Gao, Xinhao Xu, Xueyun Zheng, Yang Zhang and Xinai Zhang

Foods, 2025, 14, 3229
DOI: 10.3390/foods14183229

Rapid isothermal point-of-care test for screening of SARS-CoV-2 (COVID-19)

Jean-Marc Zingg, Yu-Ping Yang, Spencer Seely, Pratibha Joshi, Md Harun Or Roshid, Fabiola Iribarren Latasa, Gregory O'Connor, Jennifer Alfaro, Eduardo Riquelme, Sebastian Bernales, Emre Dikici, Sapna Deo and Sylvia Daunert

Aspects of Molecular Medicine, 2023, 1, 100002
DOI: 10.1016/j.amolm.2023.100002

Fully integrated and automated centrifugal microfluidic chip for point-of-care multiplexed molecular diagnostics

Yujin Xiao, Mengfan Zhou, Changgen Liu, Siyu Gao, Chao Wan, Shunji Li, Chenxi Dai, Wei Du, Xiaojun Feng, Yiwei Li, Peng Chen and Bi-Feng Liu

Biosensors and Bioelectronics, 2024, 255, 116240
DOI: 10.1016/j.bios.2024.116240

Microfluidic-based technologies for diagnosis, prevention, and treatment of COVID-19: recent advances and future directions

E. Alperay Tarim, Muge Anil Inevi, Ilayda Ozkan, Seren Kecili, Eyup Bilgi, M. Semih Baslar, Engin Ozcivici, Ceyda Oksel Karakus and H. Cumhur Tekin

Biomed Microdevices, 2023, 25
DOI: 10.1007/s10544-023-00649-z

Advances and Challenges in SARS-CoV-2 Detection: A Review of Molecular and Serological Technologies

Mai M. El-Daly

Diagnostics, 2024, 14, 519
DOI: 10.3390/diagnostics14050519

Application of microfluidic technologies on COVID-19 diagnosis and drug discovery

Zhun Lin, Zhengyu Zou, Zhe Pu, Minhao Wu and Yuanqing Zhang

Acta Pharmaceutica Sinica B, 2023, 13, 2877
DOI: 10.1016/j.apsb.2023.02.014

Application of recombinase polymerase amplification with lateral flow assay to pathogen point-of-care diagnosis

Yilian Zhao, Yan Wei, Chao Ye, Jinmeng Cao, Xiaoxing Zhou, Mengru Xie, Jilin Qing and Zhizhong Chen

Front. Cell. Infect. Microbiol., 2024, 14
DOI: 10.3389/fcimb.2024.1475922

Isothermal amplification-assisted diagnostics for COVID-19

Mariarita De Felice, Mariarosaria De Falco, Daniele Zappi, Amina Antonacci and Viviana Scognamiglio

Biosensors and Bioelectronics, 2022, 205, 114101
DOI: 10.1016/j.bios.2022.114101

A seminested recombinase polymerase amplification assay to detect rickettsial pathogens in clinical samples

Ying Zhang, Yan Hai, Biao Duan, Hu Long, Xiaofei Xie, Zhongqiu Teng, Feifei Yin, Mingliu Wang, Yanwen Xiong, Zhujun Shao, Weidong Guo and Aiping Qin

Diagnostic Microbiology and Infectious Disease, 2023, 107, 116067
DOI: 10.1016/j.diagmicrobio.2023.116067

Environmental monitoring of a climate change indicator (Vibrio vulnificus) in coastal wetland water samples based on field-deployable detection

Pedro Quintero-Campos, Rubén Salvador-Clavell, Blanca Martín, Belen Fouz, Carmen Amaro, Luis A. Tortajada-Genaro and Ángel Maquieira

Science of The Total Environment, 2025, 986, 179791
DOI: 10.1016/j.scitotenv.2025.179791

Microfluidics-based strategies for molecular diagnostics of infectious diseases

Xin Wang, Xian-Zhe Hong, Yi-Wei Li, Ying Li, Jie Wang, Peng Chen and Bi-Feng Liu

Military Med Res, 2022, 9
DOI: 10.1186/s40779-022-00374-3

Recombinase Polymerase Amplification-Based Biosensors for Rapid Zoonoses Screening

Xinrui Feng, Yan Liu, Yang Zhao, Zhe Sun, Ning Xu, Chen Zhao and Wei Xia

IJN, 2023, Volume 18, 6311
DOI: 10.2147/IJN.S434197

A compact microfluidic platform for rapid multiplex detection of respiratory viruses via centrifugal polar-absorbance spectroscopy

Ya Su, Xiangyu Jin, Fan Yang, Xuekai Liu, Fenggang Li, Qingchen Zhao, Jialu Hou, Shuailong Zhang, Hang Li, Guoliang Huang and Rongxin Fu

Talanta, 2024, 280, 126733
DOI: 10.1016/j.talanta.2024.126733

Recombinase Polymerase Amplification for Rapid Detection of Zoonotic Pathogens: An Overview

Ruichen Lv, Nianhong Lu, Junhu Wang, Yuexi Li and Yong Qi

Zoonoses, 2022, 2
DOI: 10.15212/ZOONOSES-2022-0002

fM–aM Detection of the SARS-CoV-2 Antigen by Advanced Lateral Flow Immunoassay Based on Gold Nanospheres

Yilin Liu, Li Zhan, Jesse W. Shen, Bàrbara Baro, Andrea Alemany, James Sackrison, Oriol Mitjà and John C. Bischof

ACS Appl. Nano Mater., 2021, 4, 13826
DOI: 10.1021/acsanm.1c03217

Development of a rapid point-of-care dengue virus type 2 infection diagnostic assay using recombinase polymerase amplification and lateral flow device

Meagan A. Prescott, Myra T. Koesdjojo, David T. Mandrell and Manoj K. Pastey

Front. Cell. Infect. Microbiol., 2025, 15
DOI: 10.3389/fcimb.2025.1578549

A portable thermostatic molecular diagnosis device based on high-efficiency photothermal conversion material for rapid field detection of SARS-CoV-2

Xinyao Yang, Chuanghao Guo, Qianling Zhang, Yong Chen, Yizhen Liu and Xueji Zhang

Talanta, 2023, 258, 124422
DOI: 10.1016/j.talanta.2023.124422

Microfluidics-Based Biosensing Platforms: Emerging Frontiers in Point-of-Care Testing SARS-CoV-2 and Seroprevalence

Elda A. Flores-Contreras, Reyna Berenice González-González, Iram P. Rodríguez-Sánchez, Juan F. Yee-de León, Hafiz M. N. Iqbal and Everardo González-González

Biosensors, 2022, 12, 179
DOI: 10.3390/bios12030179

Advances in Biosensing Technologies for Diagnosis of COVID-19

Sulaiman Alsalameh, Khalid Alnajjar, Tariq Makhzoum, Noor Al Eman, Ismail Shakir, Tanveer Ahmad Mir, Khaled Alkattan, Raja Chinnappan and Ahmed Yaqinuddin

Biosensors, 2022, 12, 898
DOI: 10.3390/bios12100898

An Integrated Amplification-Free Digital CRISPR/Cas-Assisted Assay for Single Molecule Detection of RNA

Dou Wang, Xuedong Wang, Feidi Ye, Jin Zou, Jiuxin Qu and Xingyu Jiang

ACS Nano, 2023, 17, 7250
DOI: 10.1021/acsnano.2c10143

Resonance-Induced Capacitively Coupled Contactless Conductivity Detection (ReC4D) Unit for Nucleic Acid Amplification Testing

Roberto G. Ramírez-Chavarría, Jorge A. Uc-Martín, Bryan E. Alvarez-Serna and Ramón F. Padilla-Morán

Technologies, 2025, 13, 138
DOI: 10.3390/technologies13040138

Portable high-throughput multimodal immunoassay platform for rapid on-site COVID-19 diagnostics

Yanke Shan, Bin Wang, Huachuan Huang, Keding Yan, Wenzhi Li, Shouyu Wang and Fei Liu

Analytica Chimica Acta, 2023, 1238, 340634
DOI: 10.1016/j.aca.2022.340634

Hollow microneedle microfluidic paper-based chip for biomolecules rapid sampling and detection in interstitial fluid

Jiale Cheng, Jinkun Huang, Qin Xiang and Haifeng Dong

Analytica Chimica Acta, 2023, 1255, 341101
DOI: 10.1016/j.aca.2023.341101

A point-of-care single nucleotide variation assay based on strand-displacement-triggered recombinase polymerase amplification

Lutan Zhang, Lulu Xu, Jian Zhang, Wenbo Wang, Yanru Huang, Yixi Zhou, Xingmei Yao, Zhaohui Liu and Yunsheng Ge

Sensors and Actuators B: Chemical, 2024, 402, 135075
DOI: 10.1016/j.snb.2023.135075

Establishment and application of the Recombinase-Aided Amplification-Lateral Flow Dipstick detection method for Pantoea ananatis on rice

Wang Aiying, Luo Ju, Wang Cilin, Hou Yuxuan, Yang Baojun, Tang Jian and Liu Shuhua

Australasian Plant Pathol., 2023, 52, 283
DOI: 10.1007/s13313-023-00918-8

Innovative technological advancements in laboratory medicine: Predicting the lab of the future

Siobhan Wilson, Shannon Steele and Khosrow Adeli

Biotechnology & Biotechnological Equipment, 2022, 36, S9
DOI: 10.1080/13102818.2021.2011413

Proof-of-concept for speedy development of rapid and simple at-home method for potential diagnosis of early COVID-19 mutant infections using nanogold and aptamer

Satheesh Ellipilli, Hongzhi Wang, Wen-Jui Lee, Dan Shu and Peixuan Guo

Nanomedicine: Nanotechnology, Biology and Medicine, 2022, 45, 102590
DOI: 10.1016/j.nano.2022.102590

Digital Recombinase Polymerase Amplification, Digital Loop‐Mediated Isothermal Amplification, and Digital CRISPR‐Cas Assisted Assay: Current Status, Challenges, and Perspectives

Weihong Yin, Jianjian Zhuang, Jiale Li, Liping Xia, Kai Hu, Juxin Yin and Ying Mu

Small, 2023, 19
DOI: 10.1002/smll.202303398

Microfluidic Device-Based Virus Detection and Quantification in Future Diagnostic Research: Lessons from the COVID-19 Pandemic

Andres Escobar, Alex Diab-Liu, Kamaya Bosland and Chang-qing Xu

Biosensors, 2023, 13, 935
DOI: 10.3390/bios13100935

Microfluidic-assisted integrated nucleic acid test strips for POCT

Nan Wang, Juan Zhang, Bin Xiao and Ailiang Chen

Talanta, 2024, 267, 125150
DOI: 10.1016/j.talanta.2023.125150

Enhanced selective discrimination of point-mutated viral RNA through false amplification regulatory direct insertion in rolling circle amplification

Hakbeom Lee, Juneseok You, Hansol Lee, Woojoo Kim, Kuewhan Jang, Jinsung Park and Sungsoo Na

Biosensors and Bioelectronics, 2024, 252, 116145
DOI: 10.1016/j.bios.2024.116145

Low-Cost Biosensor Technologies for Rapid Detection of COVID-19 and Future Pandemics

William Reis de Araujo, Heather Lukas, Marcelo D. T. Torres, Wei Gao and Cesar de la Fuente-Nunez

ACS Nano, 2024, 18, 1757
DOI: 10.1021/acsnano.3c01629

Application of recombinase polymerase amplification with CRISPR/Cas12a and multienzyme isothermal rapid amplification with lateral flow dipstick assay for Bactrocera correcta

Weisong Li, Bo Cai, Ranran Chen, Jianchen Cui, Hui Wang and Zhihong Li

Pest Management Science, 2024, 80, 3317
DOI: 10.1002/ps.8035

Palm‐Sized Lab‐In‐A‐Magnetofluidic Tube Platform for Rapid and Sensitive Virus Detection

Ziyue Li, Shuo Zhang, Jiongyu Zhang, Lori Avery, David Banach, Hui Zhao and Changchun Liu

Advanced Science, 2024, 11
DOI: 10.1002/advs.202310066

Current diagnostic approaches to detect two important betacoronaviruses: Middle East respiratory syndrome coronavirus (MERS-CoV) and severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2)

Zhi Xiong Chong, Winnie Pui Pui Liew, Hui Kian Ong, Chean Yeah Yong, Chong Seng Shit, Wan Yong Ho, Stephanie Y.L. Ng and Swee Keong Yeap

Pathology - Research and Practice, 2021, 225, 153565
DOI: 10.1016/j.prp.2021.153565

Label-free electrical monitoring of nucleic acid amplification with integrated hydrogel ionic diodes

Chenwei Xiong, Jie Li, Luyao Li, Long Chen, Rong Zhang, Xianqiang Mi and Yifan Liu

Materials Today Bio, 2022, 15, 100281
DOI: 10.1016/j.mtbio.2022.100281

A portable, rapid isothermal amplification kit enabling naked eye detection of SARS-CoV-2 RNAs

Ji-Ho Park, Kihyeun Kim, Bobin Lee, Hyungjun Jang and Min-Gon Kim

Talanta, 2025, 285, 127327
DOI: 10.1016/j.talanta.2024.127327

Fabrication of Wearable PDMS Device for Rapid Detection of Nucleic Acids via Recombinase Polymerase Amplification Operated by Human Body Heat

Kieu The Loan Trinh and Nae Yoon Lee

Biosensors, 2022, 12, 72
DOI: 10.3390/bios12020072

Photoelectrochromic sensing chip with recombinase polymerase amplification for visual detection of African swine fever virus

Ruishuang Yuan, Yinmin Min, Rui Geng, Wei Xiong, Lijun Ding and Kun Wang

Sensors and Actuators B: Chemical, 2025, 427, 137175
DOI: 10.1016/j.snb.2024.137175

A one-pot assay based on PAM-free recombinase polymerase amplification and CRISPR/Cas12a for rapid detection of SARS-CoV-2 N gene

Yixia Yang, Xinghu Ji, Fuxiang Zhou and Zhike He

Talanta, 2026, 296, 128448
DOI: 10.1016/j.talanta.2025.128448

C. difficile biomarkers, pathogenicity and detection

Sepideh Ziaei Chamgordani, Abbas Yadegar and Hedayatollah Ghourchian

Clinica Chimica Acta, 2024, 558, 119674
DOI: 10.1016/j.cca.2024.119674

Paper-Based Point-of-Care Testing of SARS-CoV-2

Yuan Jia, Hao Sun, Jinpeng Tian, Qiuming Song and Wenwei Zhang

Front. Bioeng. Biotechnol., 2021, 9
DOI: 10.3389/fbioe.2021.773304

Multi-reagents dispensing centrifugal microfluidics for point-of-care testing

Yujin Xiao, Shunji Li, Zheng Pang, Chao Wan, Lina Li, Huijuan Yuan, Xianzhe Hong, Wei Du, Xiaojun Feng, Yiwei Li, Peng Chen and Bi-Feng Liu

Biosensors and Bioelectronics, 2022, 206, 114130
DOI: 10.1016/j.bios.2022.114130

Application of nanoplasmonic biosensors based on nanoarrays in biological and chemical detection

Rui Li, Hongli Fan, Youqian Chen, Junjie Huang, Gang L. Liu and Liping Huang

Opt. Express, 2023, 31, 21586
DOI: 10.1364/OE.470786

Recombinase polymerase amplification: method’s characteristics and applications in diagnostics of infectious diseases

Olga S. Bondareva, Artem A. Baturin and Anna V. Mironova

Journal of microbiology, epidemiology and immunobiology, 2024, 101, 270
DOI: 10.36233/0372-9311-470

AI-aided on-chip nucleic acid assay for smart diagnosis of infectious disease

Hao Sun, Linghu Xiong, Yi Huang, Xinkai Chen, Yongjian Yu, Shaozhen Ye, Hui Dong, Yuan Jia and Wenwei Zhang

Fundamental Research, 2022, 2, 476
DOI: 10.1016/j.fmre.2021.12.005

Recent Progress on Rapid Lateral Flow Assay-Based Early Diagnosis of COVID-19

Ying Zhang, Yujuan Chai, Zulu Hu, Zhourui Xu, Meirong Li, Xin Chen, Chengbin Yang and Jia Liu

Front. Bioeng. Biotechnol., 2022, 10
DOI: 10.3389/fbioe.2022.866368

Nucleic acid testing of SARS-CoV-2: A review of current methods, challenges, and prospects

Yuanshou Zhu, Meng Zhang, Zhijun Jie and Shengce Tao

Front. Microbiol., 2022, 13
DOI: 10.3389/fmicb.2022.1074289

CRISPR: The frontier technology of next-generation RNA detection

Liang Zhou, Wen Xu, Jinming Kong and Xueji Zhang

Biochemical Engineering Journal, 2024, 211, 109480
DOI: 10.1016/j.bej.2024.109480

Recombinase polymerase amplification integrated with microfluidics for nucleic acid testing at point of care

Yuemeng Bai, Jingcheng Ji, Fengdan Ji, Shuang Wu, Yuan Tian, Birui Jin and Zedong Li

Talanta, 2022, 240, 123209
DOI: 10.1016/j.talanta.2022.123209

MicroRNA Sensors Based on CRISPR/Cas12a Technologies: Evolution From Indirect to Direct Detection

Songcheng Yu, Xueying Lei and Chenling Qu

Critical Reviews in Analytical Chemistry, 2025, 55, 968
DOI: 10.1080/10408347.2024.2329229

High-throughput centrifugal microfluidic platform for multiplex respiratory virus diagnostics

Hiep Van Nguyen, Vu Minh Phan and Tae Seok Seo

Sensors and Actuators B: Chemical, 2024, 399, 134771
DOI: 10.1016/j.snb.2023.134771

Microarray-based chemical sensors and biosensors: Fundamentals and food safety applications

Xiaofeng Chen, Chanyu Yao and Zheng Li

TrAC Trends in Analytical Chemistry, 2023, 158, 116785
DOI: 10.1016/j.trac.2022.116785

Recent advances of lateral flow immunoassay components as “point of need”

Susraba Chatterjee and Sumi Mukhopadhyay

Journal of Immunoassay and Immunochemistry, 2022, 43, 579
DOI: 10.1080/15321819.2022.2122063

Mechanically Compressed Barriers Improve Paper-Based Lateral Flow Assay Sensitivity for COVID-19 Nucleic Acid Detection

Nur Alam, Li Tong, Zhibin He, Ruihua Tang, Laboni Ahsan and Yonghao Ni

Ind. Eng. Chem. Res., 2023, 62, 18800
DOI: 10.1021/acs.iecr.3c02828

Recent advances and applications in integrating nucleic acid amplification technologies and CRISPR systems by spatial / temporal separation strategies for one-pot diagnosis

Di Huang, Yichen He, Chutian Xu, Peijie Shen, Shanshan Shi, Qiang Shu, Xiangming Fang and Zhinan Xu

TrAC Trends in Analytical Chemistry, 2025, 193, 118456
DOI: 10.1016/j.trac.2025.118456

Point-of-care diagnostics for sepsis using clinical biomarkers and microfluidic technology

Zoe Bradley and Nikhil Bhalla

Biosensors and Bioelectronics, 2023, 227, 115181
DOI: 10.1016/j.bios.2023.115181

Centrifugal microfluidic-based multiplex recombinase polymerase amplification assay for rapid detection of SARS-CoV-2

Ruoxu Li, Ning Su, Xiaodong Ren, Xiange Sun, Wenman Li, Yuwei Li, Jin Li, Chen Chen, Hong Wang, Weiping Lu, Shaoli Deng and Qing Huang

iScience, 2023, 26, 106245
DOI: 10.1016/j.isci.2023.106245

Novel microfluidic chips integrated with smart devices for in situ detection of foodborne pathogenic bacteria

Ruibin Hu, Wen Wang, Qingfeng Zheng, Jianhan Lin and Hua Yang

Current Opinion in Food Science, 2025, 62, 101269
DOI: 10.1016/j.cofs.2024.101269

Advances in Virus Detection Techniques Based on Recombinant Polymerase Amplification

Shiwen Wu, Wenhan Yu, Xianshu Fu, Xiaoping Yu, Zihong Ye, Mingzhou Zhang, Yulou Qiu and Biao Ma

Molecules, 2024, 29, 4972
DOI: 10.3390/molecules29204972

Portable Paper‐Based Nucleic Acid Enrichment for Field Testing

Junyang Mei, Dandan Wang, Yiheng Zhang, Dan Wu, Jinhui Cui, Mingzhe Gan and Peifeng Liu

Advanced Science, 2023, 10
DOI: 10.1002/advs.202205217

Innovative strategies for enhancing AuNP-based point-of-care diagnostics: Focus on coronavirus detection

Zhenquan Lai, Fucai Wang, Yishuang Cui, Zhaofa Li and Junsheng Lin

Talanta, 2025, 285, 127362
DOI: 10.1016/j.talanta.2024.127362

A Recombinase-Aided Amplification-Lateral Flow Dipstick Detection Technique for Early On-Site Diagnosis of Bacterial Blight Caused by Xanthomonas oryzae pv. oryzae in Rice

Yuxuan Hou, Jie Zhu, Chenglong Lu, Libo Fan, Mengqi Liang, Xiaobo Zhang, Benyi Cheng, Xia Xu and Junyi Gong

Rice Science, 2025, 32, 575
DOI: 10.1016/j.rsci.2025.01.005

Development and Efficacy of Lateral Flow Point-of-Care Testing Devices for Rapid and Mass COVID-19 Diagnosis by the Detections of SARS-CoV-2 Antigen and Anti-SARS-CoV-2 Antibodies

Wen-Yeh Hsieh, Cheng-Han Lin, Tzu-Ching Lin, Chao-Hsu Lin, Hui-Fang Chang, Chin-Hung Tsai, Hsi-Tien Wu and Chih-Sheng Lin

Diagnostics, 2021, 11, 1760
DOI: 10.3390/diagnostics11101760

Rapid Detection of SARS-CoV-2 RNA Using Reverse Transcription Recombinase Polymerase Amplification (RT-RPA) with Lateral Flow for N-Protein Gene and Variant-Specific Deletion–Insertion Mutation in S-Protein Gene

Jose L. Malaga, Monica J. Pajuelo, Michiko Okamoto, Emmanuel Kagning Tsinda, Kanako Otani, Pablo Tsukayama, Lucero Mascaro, Diego Cuicapuza, Masamichi Katsumi, Kazuhisa Kawamura, Hidekazu Nishimura, Akie Sakagami, Yo Ueki, Suguru Omiya, Satoshi Okamoto, Asami Nakayama, Shin-ichi Fujimaki, Chuyao Yu, Sikandar Azam, Eiichi Kodama, Clyde Dapat, Hitoshi Oshitani and Mayuko Saito

Viruses, 2023, 15, 1254
DOI: 10.3390/v15061254

Highly Specific and Rapid Multiplex Identification of Candida Species Using Digital Microfluidics Integrated with a Semi-Nested Genoarray

Zeyin Mao, Anni Deng, Xiangyu Jin, Tianqi Zhou, Shuailong Zhang, Meng Li, Wenqi Lv, Leyang Huang, Hao Zhong, Shihong Wang, Yixuan Shi, Lei Zhang, Qinping Liao, Rongxin Fu and Guoliang Huang

Anal. Chem., 2024, 96, 18797
DOI: 10.1021/acs.analchem.4c04265

PAM-free loop-mediated isothermal amplification coupled with CRISPR/Cas12a cleavage (Cas-PfLAMP) for rapid detection of rice pathogens

Zaobing Zhu, Rong Li, Hanwen Zhang, Jinyue Wang, Yongyi Lu, Dabing Zhang and Litao Yang

Biosensors and Bioelectronics, 2022, 204, 114076
DOI: 10.1016/j.bios.2022.114076

Self-assembly of DNA-gold nanoaggregate for visual detection of thymidine kinase 1 (TK1) mRNA via lateral flow assay

Jing Wang, Lu Shi, Qiaorong Tang, Xinyu Zhu, Mengmeng Wu, Wei Liu, Baoxin Li and Yan Jin

Microchim Acta, 2023, 190
DOI: 10.1007/s00604-023-06036-y

RT-RPA and RPA-LFA assay for rapid and ultrasensitive detection of Vibrio parahaemolyticus

Antuo Hu, Huan Chen, Changzheng Shi, Zhaoxin Lu, Fanqiang Meng and Xiaomei Bie

Food Control, 2024, 166, 110732
DOI: 10.1016/j.foodcont.2024.110732

Smartphone-readable RPA-LFA for the high-sensitivity detection of Leishmania kDNA using nanophosphor reporters

Adheesha N. Danthanarayana, Suman Nandy, Katerina Kourentzi, Binh Vu, Thomas R. Shelite, Bruno L. Travi, Jakoah Brgoch, Richard C. Willson and Johan van Griensven

PLoS Negl Trop Dis, 2023, 17, e0011436
DOI: 10.1371/journal.pntd.0011436

Fast, Simple Electrochromic Biosensors for Point-of-Use Infectious Disease Diagnosis

Songyi Yeon, Marjon Zamani, Xingcheng Zhou and Ariel L. Furst

Anal. Chem., 2025, 97, 17184
DOI: 10.1021/acs.analchem.5c03406

A real-time fluorogenic recombinase polymerase amplification microfluidic chip (on-chip RPA) for multiple detection of pathogenic microorganisms of penaeid shrimp

Qiang Li, Lijun Duan, Dongsheng Jin, Yuxin Chen, Yirong Lou, Qianjin Zhou, Zhongjie Xu, Fangjie Chen, Hongxian Chen, Guizong Xu, Maocang Yan, Guanjun Yang, Jianfei Lu, Yanjun Zhang and Jiong Chen

Aquaculture, 2024, 578, 740017
DOI: 10.1016/j.aquaculture.2023.740017

Advanced Point‐of‐Care Testing Technologies for Human Acute Respiratory Virus Detection

Zhaowei Zhang, Peng Ma, Rajib Ahmed, Jie Wang, Demir Akin, Fernando Soto, Bi‐Feng Liu, Peiwu Li and Utkan Demirci

Advanced Materials, 2022, 34
DOI: 10.1002/adma.202103646

A fully-enclosed prototype ‘pen’ for rapid detection of SARS-CoV-2 based on RT-RPA with dipstick assay at point-of-care testing

Qiang Fu, Yunping Tu, Lun Cheng, Lulu Zhang and Xianbo Qiu

Sensors and Actuators B: Chemical, 2023, 383, 133531
DOI: 10.1016/j.snb.2023.133531

Integrated microfluidic devices for in vitro diagnostics at point of care

Dan Liu, Ying Wang, Xingrui Li, Mengmeng Li, Qiaoyi Wu, Yanling Song, Zhi Zhu and Chaoyong Yang

Aggregate, 2022, 3
DOI: 10.1002/agt2.184

Isothermal Recombinase Polymerase Amplification (RPA) of E. coli gDNA in Commercially Fabricated PCB-Based Microfluidic Platforms

Maria Georgoutsou-Spyridonos, Myrto Filippidou, Georgia D. Kaprou, Dimitrios C. Mastellos, Stavros Chatzandroulis and Angeliki Tserepi

Micromachines, 2021, 12, 1387
DOI: 10.3390/mi12111387

Advances in Nucleic Acid Amplification-Based Microfluidic Devices for Clinical Microbial Detection

Thi Ngoc Diep Trinh and Nae Yoon Lee

Chemosensors, 2022, 10, 123
DOI: 10.3390/chemosensors10040123

Multiplex Assay of Viruses Integrating Recombinase Polymerase Amplification, Barcode—Anti-Barcode Pairs, Blocking Anti-Primers, and Lateral Flow Assay

Alexandr V. Ivanov, Irina V. Safenkova, Anatoly V. Zherdev and Boris B. Dzantiev

Anal. Chem., 2021, 93, 13641
DOI: 10.1021/acs.analchem.1c03030

Microfluidics-Based Point-of-Care Testing (POCT) Devices in Dealing with Waves of COVID-19 Pandemic: The Emerging Solution

Avinash Kumar, Arpana Parihar, Udwesh Panda and Dipesh Singh Parihar

ACS Appl. Bio Mater., 2022, 5, 2046
DOI: 10.1021/acsabm.1c01320

An Integrated Nucleic Acid Detection Method Based on Microfluidic Chip for Collection and Culture of Rice False Smut Spores

Ning Yang, Yuanyuan Ji, Aiying Wang, Jian Tang, Shuhua Liu, Xiaodong Zhang, Lijia Xu and Yong He

SSRN Journal, 2022
DOI: 10.2139/ssrn.4110832

Recent advances in recombinase polymerase amplification: Principle, advantages, disadvantages and applications

Meiying Tan, Chuan Liao, Lina Liang, Xueli Yi, Zihan Zhou and Guijiang Wei

Front. Cell. Infect. Microbiol., 2022, 12
DOI: 10.3389/fcimb.2022.1019071

SARS-CoV-2 Diagnostics Based on Nucleic Acids Amplification: From Fundamental Concepts to Applications and Beyond

João M. Vindeirinho, Eva Pinho, Nuno F. Azevedo and Carina Almeida

Front. Cell. Infect. Microbiol., 2022, 12
DOI: 10.3389/fcimb.2022.799678

One-pot, ultrasensitive, and multiplex detection of SARS-CoV-2 genes utilizing self-priming hairpin-mediated isothermal amplification

Yan Li, Taejoon Kang and Hyun Gyu Park

Biosensors and Bioelectronics, 2023, 237, 115522
DOI: 10.1016/j.bios.2023.115522

Rapid and simple detection of Phytophthora cactorum in strawberry using a coupled recombinase polymerase amplification–lateral flow strip assay

Xinyu Lu, Heng Xu, Wen Song, Zitong Yang, Jia Yu, Yuee Tian, Min Jiang, Danyu Shen and Daolong Dou

Phytopathol Res, 2021, 3
DOI: 10.1186/s42483-021-00089-8

Combining recombinase polymerase amplification and DNA‐templated reaction for SARS‐CoV‐2 sensing with dual fluorescence and lateral flow assay output

Lluc Farrera‐Soler, Arthur Gonse, Ki Tae Kim, Sofia Barluenga and Nicolas Winssinger

Biopolymers, 2022, 113
DOI: 10.1002/bip.23485

Paper‐Based Bacterial Lysis Enables Sample‐to‐Answer Home‐based DNA Testing

Georgios Chondrogiannis, Pedro Réu and Mahiar M. Hamedi

Adv Materials Technologies, 2023, 8
DOI: 10.1002/admt.202201004

Multiple functionalities of functional nucleic acids for developing high-performance lateral flow assays

Jia Wang, Longjiao Zhu, Tianshun Li, Xiangyang Li, Kunlun Huang and Wentao Xu

TrAC Trends in Analytical Chemistry, 2022, 148, 116529
DOI: 10.1016/j.trac.2022.116529

Visual detection of Mycobacterium tuberculosis in exhaled breath using N95 enrichment respirator, RPA, and lateral flow assay

Jie Cheng, Yiwei An, Qiyou Wang, Zuanguang Chen and Yanli Tong

Talanta, 2025, 286, 127490
DOI: 10.1016/j.talanta.2024.127490

Simultaneous Rapid Nucleic Acid and Protein Detection in a Lateral Chromatography Chip for COVID-19 Diagnosis

Qiuyuan Lin, Jin Zhang, Liling Liu, Jilie Kong and Xueen Fang

ACS Omega, 2022, 7, 38409
DOI: 10.1021/acsomega.2c03499

Biomedical Applications of Microfluidic Devices: A Review

Ghazaleh Gharib, İsmail Bütün, Zülâl Muganlı, Gül Kozalak, İlayda Namlı, Seyedali Seyedmirzaei Sarraf, Vahid Ebrahimpour Ahmadi, Erçil Toyran, Andre J. van Wijnen and Ali Koşar

Biosensors, 2022, 12, 1023
DOI: 10.3390/bios12111023

Rapid on-site nucleic acid testing: On-chip sample preparation, amplification, and detection, and their integration into all-in-one systems

Jingwen Wang, Han Jiang, Leiming Pan, Xiuying Gu, Chaogeng Xiao, Pengpeng Liu, Yulong Tang, Jiehong Fang, Xiaoqian Li and Chenze Lu

Front. Bioeng. Biotechnol., 2023, 11
DOI: 10.3389/fbioe.2023.1020430

Simple, Visual, Point-of-Care SARS-CoV-2 Detection Incorporating Recombinase Polymerase Amplification and Target DNA–Protein Crosslinking Enhanced Chemiluminescence

Hui Chen, Zhiyuan Zhuang, Naihan Xu, Ying Feng, Kaixin Fang, Chunyan Tan and Ying Tan

Biosensors, 2024, 14, 135
DOI: 10.3390/bios14030135

Establishment of recombinase polymerase amplification detection method for Dactylobotrys graminicola

Haiqing Zhang, Liyifan Chen, Ruifang Dong, Haowen Zheng, Lu Hou and Qiang Yao

Sci Rep, 2024, 14
DOI: 10.1038/s41598-024-76921-w

Capillarity-powered and CRISPR/Cas12a-responsive DNA hydrogel distance sensor for highly sensitive visual detection of HPV DNA

Hongru Pian, Hui Wang, Honghong Wang, Fu Tang and Zhengping Li

Biosensors and Bioelectronics, 2024, 264, 116657
DOI: 10.1016/j.bios.2024.116657

CRISPR/Cas9 bridged recombinase polymerase amplification with lateral flow biosensor removing potential primer-dimer interference for robust Staphylococcus aureus assay

Haoyu Wang, Qian Wu, Chao Yan, Jianguo Xu, Xinsheng Qin, Jie Wang, Wei Chen, Li Yao, Lin Huang and Panzhu Qin

Sensors and Actuators B: Chemical, 2022, 369, 132293
DOI: 10.1016/j.snb.2022.132293

Advances in Nucleic Acid Assays for Infectious Disease: The Role of Microfluidic Technology

Yiran Wang, Jingwei Chen, Zhijin Yang, Xuanyu Wang, Yule Zhang, Mengya Chen, Zizhen Ming, Kaihuan Zhang, Dawei Zhang and Lulu Zheng

Molecules, 2024, 29, 2417
DOI: 10.3390/molecules29112417

Microfluidics for COVID-19: From Current Work to Future Perspective

Qi Li, Xingchen Zhou, Qian Wang, Wenfang Liu and Chuanpin Chen

Biosensors, 2023, 13, 163
DOI: 10.3390/bios13020163

Advancing Nematode Identification on Potato: An Isothermal Recombinase Polymerase Amplification Assay for Stubby Root Nematode, Paratrichodorus allius

Mankanwal Goraya, Guiping Yan, Jonathan Whitworth and Kylie Swisher Grimm

Am. J. Potato Res., 2024, 101, 52
DOI: 10.1007/s12230-023-09940-4

Direct Quantitation of SARS‐CoV‐2 Virus in Urban Ambient Air via a Continuous‐Flow Electrochemical Bioassay

Fuze Jiang, Bei Liu, Yang Yue, Yile Tao, Zhen Xiao, Meng Li, Zheng Ji, Jiukai Tang, Guangyu Qiu, Martin Spillmann, Junji Cao, Lianjun Zhang and Jing Wang

Advanced Science, 2023, 10
DOI: 10.1002/advs.202301222

Wearable nucleic acid testing platform - A perspective on rapid self-diagnosis and surveillance of infectious diseases

Gokul Chandra Biswas, Md. Taufiqur Mannan Khan and Jagotamoy Das

Biosensors and Bioelectronics, 2023, 226, 115115
DOI: 10.1016/j.bios.2023.115115

DNA‐Programmed Reaction to Evaluate Specific IgE for Allergy Point‐of‐Care Testing

Yan Shan Ang, Dionne Ker Xin Low and Lin‐Yue Lanry Yung

Small, 2025, 21
DOI: 10.1002/smll.202500575

Recent advances of functional nucleic acid-based sensors for point-of-care detection of SARS-CoV-2

Wenxian Zhang, Ying He, Zhe Feng and Jingjing Zhang

Microchim Acta, 2022, 189
DOI: 10.1007/s00604-022-05242-4

Recent progress in microfluidic biosensors with different driving forces

Yilong Liu, Haicong Shen, Xiaoping Yang, Siyin Kang, Linfeng Cai, Tian Tian, Rui Su, Chaoyong Yang and Zhi Zhu

TrAC Trends in Analytical Chemistry, 2023, 158, 116894
DOI: 10.1016/j.trac.2022.116894

CESSAT: A chemical additive-enhanced single-step accurate CRISPR/Cas13 testing system for field-deployable ultrasensitive detection and genotyping of SARS-CoV-2 variants of concern

Yunxiang Wang, Hong Chen, Huixia Gao, Hongjuan Wei, Yuling Wang, Kai Mu, Liyan Liu, Erhei Dai, Zhen Rong and Shengqi Wang

Biosensors and Bioelectronics, 2023, 229, 115238
DOI: 10.1016/j.bios.2023.115238

Dual-Gene Isothermal Amplification Coupled with Lateral Flow Strip for On-Site Accurate Detection of E. coli O157:H7 in Food Samples

Ting Zheng, Xianming Li, Ya-Ni Xie, Bin Yang and Peng Wu

Anal. Chem., 2023, 95, 6053
DOI: 10.1021/acs.analchem.3c00141

Multiplex microRNA detection for breast cancer precise diagnosis by constructing split crRNA-based amplification-free CRISPR/Cas12a biosensor

Yeling Yang, Yufei Yang, Xuhua Mao, Wenhua Zhou, Xue-Feng Yu, Guobin Mao and Yingxin Ma

Chemical Engineering Journal, 2025, 522, 167489
DOI: 10.1016/j.cej.2025.167489