Brian J. O'Grady, Michael D. Geuy, Hyosung Kim, Kylie M. Balotin, Everett R. Allchin, David C. Florian, Neelansh N. Bute, Taylor E. Scott, Gregory B. Lowen, Colin M. Fricker, Matthew L. Fitzgerald, Scott A. Guelcher, John P. Wikswo, Leon M. Bellan and Ethan S. Lippmann

Lab Chip, 2021, 21, 4814
DOI: 10.1039/D1LC00744K

Microfluidic technologies for lipid vesicle generation

Yu Cheng, Callum D. Hay, Suchaya M. Mahuttanatan, James W. Hindley, Oscar Ces and Yuval Elani

Lab Chip, 2024, 24, 4679
DOI: 10.1039/D4LC00380B

One step generation of single-core double emulsions from polymer-osmose-induced aqueous phase separation in polar oil droplets

Jean-Paul Douliez, Anais Arlaut, Laure Beven, Anne-Laure Fameau and Arnaud Saint-Jalmes

Soft Matter, 2023, 19, 7562
DOI: 10.1039/D3SM00970J

Permeation Studies across Symmetric and Asymmetric Membranes in Microdroplet Arrays

Simon Bachler, Marion Ort, Stefanie D. Krämer and Petra S. Dittrich

Anal. Chem., 2021, 93, 5137
DOI: 10.1021/acs.analchem.0c04939

Fusing Artificial Cell Compartments and Lipid Domains Using Optical Traps: A Tool to Modulate Membrane Composition and Phase Behaviour

Adithya Vivek, Guido Bolognesi and Yuval Elani

Micromachines, 2020, 11, 388
DOI: 10.3390/mi11040388

Pushbutton-activated microfluidic cartridge as a user-friendly sample preparation tool for diagnostics

Juhwan Park and Je-Kyun Park

Biomicrofluidics, 2021, 15
DOI: 10.1063/5.0056580

Microfluidic production of monodisperse emulsions for cosmetics

Daehwan Park, Hajeong Kim and Jin Woong Kim

Biomicrofluidics, 2021, 15
DOI: 10.1063/5.0057733

Acoustics-Controlled Microdroplet and Microbubble Fusion and Its Application in the Synthesis of Hydrogel Microspheres

Shaobo Jin, Guoyong Ye, Na Cao, Xuling Liu, Liguo Dai, Pengpeng Wang, Tong Wang and Xueyong Wei

Langmuir, 2022, 38, 12602
DOI: 10.1021/acs.langmuir.2c02080

Engineering consortia by polymeric microbial swarmbots

Lin Wang, Xi Zhang, Chenwang Tang, Pengcheng Li, Runtao Zhu, Jing Sun, Yunfeng Zhang, Hua Cui, Jiajia Ma, Xinyu Song, Weiwen Zhang, Xiang Gao, Xiaozhou Luo, Lingchong You, Ye Chen and Zhuojun Dai

Nat Commun, 2022, 13
DOI: 10.1038/s41467-022-31467-1

One-step preparation of photoresponsive microcapsules based on the interfacial self-assembly of an azopyridine coordination polymer

Jian Wang, Botian Li, Xianxiao Song, Xueyan Liu, Da Xiao, Yichi Zhang, Jundang Liang and Jianhong Xu

Chemical Engineering Journal, 2023, 463, 142403
DOI: 10.1016/j.cej.2023.142403

Label‐free monitoring and manipulation of microfluidic water‐in‐oil droplets

Christoph Frey, Jonas Pfeil, Tobias Neckernuss, Daniel Geiger, Klaus Weishaupt, Ilia Platzman, Othmar Marti and Joachim P. Spatz

VIEW, 2020, 1
DOI: 10.1002/VIW.20200101

Interfacing Living and Synthetic Cells as an Emerging Frontier in Synthetic Biology

Yuval Elani

Angew Chem Int Ed, 2021, 60, 5602
DOI: 10.1002/anie.202006941

How does the Internet of Things (IoT) help in microalgae biorefinery?

Kexin Wang, Kuan Shiong Khoo, Hui Yi Leong, Dillirani Nagarajan, Kit Wayne Chew, Huong Yong Ting, Anurita Selvarajoo, Jo-Shu Chang and Pau Loke Show

Biotechnology Advances, 2022, 54, 107819
DOI: 10.1016/j.biotechadv.2021.107819

The Usual Suspects 2019: of Chips, Droplets, Synthesis, and Artificial Cells

Christoph Eilenberger, Sarah Spitz, Barbara Bachmann, Eva Ehmoser, Peter Ertl and Mario Rothbauer

Micromachines, 2019, 10, 285
DOI: 10.3390/mi10050285

Micro-Surface and -Interfacial Tensions Measured Using the Micropipette Technique: Applications in Ultrasound-Microbubbles, Oil-Recovery, Lung-Surfactants, Nanoprecipitation, and Microfluidics

David Needham, Koji Kinoshita and Anders Utoft

Micromachines, 2019, 10, 105
DOI: 10.3390/mi10020105

Recent advances of droplet-based microfluidics for engineering artificial cells

Samantha Fasciano and Shue Wang

SLAS Technology, 2024, 29, 100090
DOI: 10.1016/j.slast.2023.05.002

Synthetic Cells Revisited: Artificial Cell Construction Using Polymeric Building Blocks

Viviana Maffeis, Lukas Heuberger, Anamarija Nikoletić, Cora‐Ann Schoenenberger and Cornelia G. Palivan

Advanced Science, 2024, 11
DOI: 10.1002/advs.202305837

Multicompartment Synthetic Vesicles for Artificial Cell Applications

Jooyong Shin and Yeongseon Jang

ACS Appl. Eng. Mater., 2024, 2, 2758
DOI: 10.1021/acsaenm.3c00812

A comprehensive review of Microfluidic approaches in cell-free synthetic biology

Amogh Kumar Baranwal and Sebastian J. Maerkl

Front. Synth. Biol., 2024, 2
DOI: 10.3389/fsybi.2024.1397533

Fluorinated oil-surfactant mixtures with the density of water: Artificial cells for synthetic biology

Roberto Laos, Steven Benner and Floyd Romesberg

PLoS ONE, 2022, 17, e0252361
DOI: 10.1371/journal.pone.0252361

Lipid‐Hybrid Multicompartment Membrane Systems for Controlled, Compartmentalized Encapsulant Release

Tsuyoshi Inaba, Richard J Archer, David A Gregory and Shin‐ichiro M Nomura

Adv Materials Inter, 2025, 12
DOI: 10.1002/admi.202400959

Microfluidic platform enables tailored translocation and reaction cascades in nanoliter droplet networks

Simon Bachler, Dominik Haidas, Marion Ort, Todd A. Duncombe and Petra S. Dittrich

Commun Biol, 2020, 3
DOI: 10.1038/s42003-020-01489-w

A microfluidic platform for the controlled synthesis of architecturally complex liquid crystalline nanoparticles

Colin P. Pilkington, Claudia Contini, Joseph D. Barritt, Paul A. Simpson, John M. Seddon and Yuval Elani

Sci Rep, 2023, 13
DOI: 10.1038/s41598-023-39205-3

Microfluidics for Biosynthesizing: from Droplets and Vesicles to Artificial Cells

Yongjian Ai, Ruoxiao Xie, Jialiang Xiong and Qionglin Liang

Small, 2020, 16
DOI: 10.1002/smll.201903940

ESCRT-III induces phase separation in model membranes prior to budding and causes invagination of the liquid-ordered phase

Yunuen Avalos-Padilla, Vasil N. Georgiev and Rumiana Dimova

Biochimica et Biophysica Acta (BBA) - Biomembranes, 2021, 1863, 183689
DOI: 10.1016/j.bbamem.2021.183689

Microfluidic fabrication of novel polymeric core‐shell microcapsules for storage of CO2 solvents and organic chelating agents

Yuan Zhao, Seyedarash Moshtaghibana, Tianle Zhu, Kehinde A. Fayemiwo, Adam Price and Goran T. Vladisavljević

Journal of Polymer Science, 2022, 60, 1727
DOI: 10.1002/pol.20210959

Biophysical quantification of unitary solute and solvent permeabilities to enable translation to membrane science

Johann Wachlmayr, Laxmicharan Samineni, Denis G. Knyazev, Thomas Barta, Armin Speletz, Chenhao Yao, Hyeonji Oh, Harekrushna Behera, Tingwei Ren, Manish Kumar and Andreas Horner

Journal of Membrane Science, 2023, 675, 121308
DOI: 10.1016/j.memsci.2022.121308

Combining 3D Printing and Microfluidic Techniques: A Powerful Synergy for Nanomedicine

Federica Sommonte, Nunzio Denora and Dimitrios A. Lamprou

Pharmaceuticals, 2023, 16, 69
DOI: 10.3390/ph16010069

Kompartimentäre Polyampholyt‐Mikrogele mittels entropisch bedingter Ausflockung und Koazervation von Nanogelen in Emulsionströpfchen

Hannah F. Mathews, Maria I. Pieper, Se‐Hyeong Jung and Andrij Pich

Angewandte Chemie, 2023, 135
DOI: 10.1002/ange.202304908

Direct manipulation of liquid ordered lipid membrane domains using optical traps

Mark S. Friddin, Guido Bolognesi, Ali Salehi-Reyhani, Oscar Ces and Yuval Elani

Commun Chem, 2019, 2
DOI: 10.1038/s42004-018-0101-4

Microfluidics: Innovations in Materials and Their Fabrication and Functionalization

Jacob B. Nielsen, Robert L. Hanson, Haifa M. Almughamsi, Chao Pang, Taylor R. Fish and Adam T. Woolley

Anal. Chem., 2020, 92, 150
DOI: 10.1021/acs.analchem.9b04986

Hydrogels as functional components in artificial cell systems

Matthew E. Allen, James W. Hindley, Divesh K. Baxani, Oscar Ces and Yuval Elani

Nat Rev Chem, 2022, 6, 562
DOI: 10.1038/s41570-022-00404-7

Numerical investigations of electric field-mediated active droplet transfer in a ternary phase system

Satarupa Dutta, Partho Sarathi Gooh Pattader and Dipankar Bandyopadhyay

Physics of Fluids, 2022, 34
DOI: 10.1063/5.0104912

Programmable pulsed aerodynamic printing for multi-interface composite manufacturing

Zhiqiang Zhu, Tianao Chen, Yuanqing Zhu, Fangsheng Huang, Kai Mu, Ting Si and Ronald X. Xu

Matter, 2023, 6, 2034
DOI: 10.1016/j.matt.2023.04.017

Liposomes like advanced drug carriers: from fundamentals to pharmaceutical applications

Huma Hameed, Mahtab Ahmad Khan, Ana Cláudia Paiva-Santos, Saleha Faheem, Aleena Khalid, Muhammad Sohaib Majid, Aiman Adnan and Fizza Rana

Journal of Microencapsulation, 2024, 41, 456
DOI: 10.1080/02652048.2024.2376116

dCITI-Seq: droplet combinational indexed transposon insertion sequencing

Jing Tu, Yi Qiao, Zheyun Xu, Na Lu, Naiyun Long and Zuhong Lu

Anal Bioanal Chem, 2022, 414, 2661
DOI: 10.1007/s00216-022-03902-1

Emerging Advances in Microfluidic Hydrogel Droplets for Tissue Engineering and STEM Cell Mechanobiology

Mohamad Orabi and Joe F. Lo

Gels, 2023, 9, 790
DOI: 10.3390/gels9100790

Microfluidic Handling and Analysis of Giant Vesicles for Use as Artificial Cells: A Review

Tom Robinson

Adv. Biosys., 2019, 3
DOI: 10.1002/adbi.201800318

Manufacture of Multilayered Artificial Cell Membranes through Sequential Bilayer Deposition on Emulsion Templates

Tsoi Ip, Qien Li, Nick Brooks and Yuval Elani

ChemBioChem, 2021, 22, 2275
DOI: 10.1002/cbic.202100072

Rapid prototyping of microfluidics devices using novel thermoset polydicyclopentadiene

Guo Song, Yang Weicheng and Luo Yong

J. Micromech. Microeng., 2023, 33, 075002
DOI: 10.1088/1361-6439/acd25c

Scalable Synthesis of Planar Macroscopic Lipid-Based Multi-Compartment Structures

Richard J. Archer, Shogo Hamada, Ryo Shimizu and Shin-Ichiro M. Nomura

Langmuir, 2023, 39, 4863
DOI: 10.1021/acs.langmuir.2c02859

Toward Artificial Cells: Novel Advances in Energy Conversion and Cellular Motility

Sungwoo Jeong, Huong Thanh Nguyen, Chang Ho Kim, Mai Nguyet Ly and Kwanwoo Shin

Adv Funct Materials, 2020, 30
DOI: 10.1002/adfm.201907182

Multiphase flow in microfluidics: From droplets and bubbles to the encapsulated structures

Amirmohammad Sattari, Pedram Hanafizadeh and Mina Hoorfar

Advances in Colloid and Interface Science, 2020, 282, 102208
DOI: 10.1016/j.cis.2020.102208

Enhanced Catalytic Activity of Gold@Polydopamine Nanoreactors with Multi-compartment Structure Under NIR Irradiation

Shilin Mei, Zdravko Kochovski, Rafael Roa, Sasa Gu, Xiaohui Xu, Hongtao Yu, Joachim Dzubiella, Matthias Ballauff and Yan Lu

Nano-Micro Lett., 2019, 11
DOI: 10.1007/s40820-019-0314-9

Manipulation of encapsulated artificial phospholipid membranes using sub-micellar lysolipid concentrations

Pantelitsa Dimitriou, Jin Li, William David Jamieson, Johannes Josef Schneider, Oliver Kieran Castell and David Anthony Barrow

Commun Chem, 2024, 7
DOI: 10.1038/s42004-024-01209-z

Compartmentalized Polyampholyte Microgels by Depletion Flocculation and Coacervation of Nanogels in Emulsion Droplets

Hannah F. Mathews, Maria I. Pieper, Se‐Hyeong Jung and Andrij Pich

Angew Chem Int Ed, 2023, 62
DOI: 10.1002/anie.202304908

Microfluidic Formation of Honeycomb-Patterned Droplets Bounded by Interface Bilayers via Bimodal Molecular Adsorption

Shougo Fujiwara, Kan Shoji, Chiho Watanabe, Ryuji Kawano and Miho Yanagisawa

Micromachines, 2020, 11, 701
DOI: 10.3390/mi11070701

Free‐Boundary Microfluidic Platform for Advanced Materials Manufacturing and Applications

Zhiqiang Zhu, Tianao Chen, Fangsheng Huang, Shiyu Wang, Pingan Zhu, Ronald X. Xu and Ting Si

Advanced Materials, 2024, 36
DOI: 10.1002/adma.202304840

Experimental study on dynamics of double emulsion droplets flowing through the Y-shaped bifurcation

Zhaomiao Liu, Wenchao Liu, Yan Pang, Dezhi Li, Chenchen Zhang and Xiang Wang

Colloids and Surfaces A: Physicochemical and Engineering Aspects, 2022, 654, 130141
DOI: 10.1016/j.colsurfa.2022.130141

Engineering spatiotemporal organization and dynamics in synthetic cells

Alessandro Groaz, Hossein Moghimianavval, Franco Tavella, Tobias W. Giessen, Anthony G. Vecchiarelli, Qiong Yang and Allen P. Liu

WIREs Nanomed Nanobiotechnol, 2021, 13
DOI: 10.1002/wnan.1685

CO2 Laser Fabrication of PMMA Microfluidic Double T-Junction Device with Modified Inlet-Angle for Cost-Effective PCR Application

Gamal A. Nasser, Ahmed M.R. Fath El-Bab, Ahmed L. Abdel-Mawgood, Hisham Mohamed and Abdelatty M. Saleh

Micromachines, 2019, 10, 678
DOI: 10.3390/mi10100678

Flow patterns and dynamic mechanisms of immiscible fluids in cross junctions with different aspect ratios

Xiang Wang, Yilin Ma, Chenchen Zhang, Yan Pang and Zhaomiao Liu

Experimental Thermal and Fluid Science, 2023, 149, 111019
DOI: 10.1016/j.expthermflusci.2023.111019

Polymersomes as the Next Attractive Generation of Drug Delivery Systems: Definition, Synthesis and Applications

Mariana Fonseca, Ivana Jarak, Francis Victor, Cátia Domingues, Francisco Veiga and Ana Figueiras

Materials, 2024, 17, 319
DOI: 10.3390/ma17020319

Droplet Microfluidics for High-Throughput Screening and Directed Evolution of Biomolecules

Goran Vladisavljević

Micromachines, 2024, 15, 971
DOI: 10.3390/mi15080971

Effects of channel geometry and physicochemical properties of solutions on stable double emulsion production in planar microfluidic devices having triangular orifices

Ruri Hidema, Ryotaro Ohashi, Susan J. Muller and Hiroshi Suzuki

AIP Advances, 2021, 11
DOI: 10.1063/5.0055436

Dynamic Synthetic Cells Based on Liquid–Liquid Phase Separation

Nicolas Martin

ChemBioChem, 2019, 20, 2553
DOI: 10.1002/cbic.201900183

Biomimetic Materials to Fabricate Artificial Cells

Haisheng Peng, Man Zhao, Xiaoying Liu, Tianjian Tong, Wenyuan Zhang, Chen Gong, Ratul Chowdhury and Qun Wang

Chem. Rev., 2024, 124, 13178
DOI: 10.1021/acs.chemrev.4c00241

Functional Interactions Between Bottom‐Up Synthetic Cells and Living Matter for Biomedical Applications

Marleen H. M. E. van Stevendaal, Jan C. M. van Hest and Alexander F. Mason

ChemSystemsChem, 2021, 3
DOI: 10.1002/syst.202100009

Is Research on “Synthetic Cells” Moving to the Next Level?

Pasquale Stano

Life, 2018, 9, 3
DOI: 10.3390/life9010003

Optoelectronic Manipulation, Trapping, Splitting, and Merging of Water Droplets and Aqueous Biodroplets Based on the Bulk Photovoltaic Effect

Andrés Puerto, Angel Méndez, Luis Arizmendi, Angel García-Cabañes and Mercedes Carrascosa

Phys. Rev. Applied, 2020, 14
DOI: 10.1103/PhysRevApplied.14.024046

Bending Rigidity, Capacitance, and Shear Viscosity of Giant Vesicle Membranes Prepared by Spontaneous Swelling, Electroformation, Gel-Assisted, and Phase Transfer Methods: A Comparative Study

Hammad A. Faizi, Annie Tsui, Rumiana Dimova and Petia M. Vlahovska

Langmuir, 2022, 38, 10548
DOI: 10.1021/acs.langmuir.2c01402

Giant Vesicles Produced with Phosphatidylcholines (PCs) and Phosphatidylethanolamines (PEs) by Water-in-Oil Inverted Emulsions

Boying Xu, Jinquan Ding, Jian Xu and Tetsuya Yomo

Life, 2021, 11, 223
DOI: 10.3390/life11030223

Measuring Encapsulation Efficiency in Cell-Mimicking Giant Unilamellar Vesicles

Pashiini Supramaniam, Zibo Wang, Stelios Chatzimichail, Christopher Parperis, Aditi Kumar, Vanessa Ho, Oscar Ces and Ali Salehi-Reyhani

ACS Synth. Biol., 2023, 12, 1227
DOI: 10.1021/acssynbio.2c00684

Development of Artificial Cell Models Using Microfluidic Technology and Synthetic Biology

Koki Kamiya

Micromachines, 2020, 11, 559
DOI: 10.3390/mi11060559

Research Progress of Phospholipid Vesicles in Biological Field

Na Zhang, Jie Song and Yuchun Han

Biomolecules, 2024, 14, 1628
DOI: 10.3390/biom14121628

Asynchronous generation of oil droplets using a microfluidic flow focusing system

Peter Thurgood, Sara Baratchi, Aram Arash, Elena Pirogova, Aaron R. Jex and Khashayar Khoshmanesh

Sci Rep, 2019, 9
DOI: 10.1038/s41598-019-47078-8

Microfluidics for High Throughput Screening of Biological Agents and Therapeutics

Anant Bhusal, Swaprakash Yogeshwaran, Hossein Goodarzi Hosseinabadi, Berivan Cecen and Amir K. Miri

Biomedical Materials & Devices, 2025, 3, 93
DOI: 10.1007/s44174-024-00169-1

Compartmentalizing Cell-Free Systems: Toward Creating Life-Like Artificial Cells and Beyond

Eunhee Cho and Yuan Lu

ACS Synth. Biol., 2020, 9, 2881
DOI: 10.1021/acssynbio.0c00433

New opportunities for creating man-made bioarchitectures utilizing microfluidics

Samar Damiati

Biomed Microdevices, 2019, 21
DOI: 10.1007/s10544-019-0415-8

Directed Signaling Cascades in Monodisperse Artificial Eukaryotic Cells

Sunidhi C. Shetty, Naresh Yandrapalli, Kerstin Pinkwart, Dorothee Krafft, Tanja Vidakovic-Koch, Ivan Ivanov and Tom Robinson

ACS Nano, 2021, 15, 15656
DOI: 10.1021/acsnano.1c04219

New Directions for Artificial Cells Using Prototyped Biosystems

Mark S. Friddin, Yuval Elani, Tatiana Trantidou and Oscar Ces

Anal. Chem., 2019, 91, 4921
DOI: 10.1021/acs.analchem.8b04885

Protocells: Milestones and Recent Advances

Irep Gözen, Elif Senem Köksal, Inga Põldsalu, Lin Xue, Karolina Spustova, Esteban Pedrueza‐Villalmanzo, Ruslan Ryskulov, Fanda Meng and Aldo Jesorka

Small, 2022, 18
DOI: 10.1002/smll.202106624

Interfacing Living and Synthetic Cells as an Emerging Frontier in Synthetic Biology

Yuval Elani

Angewandte Chemie, 2021, 133, 5662
DOI: 10.1002/ange.202006941

Functionalisation of human chloride intracellular ion channels in microfluidic droplet-interface-bilayers

Yu Zhang, Hazel Bracken, Cheryl Woolhead and Michele Zagnoni

Biosensors and Bioelectronics, 2020, 150, 111920
DOI: 10.1016/j.bios.2019.111920

On‐Chip De Novo Production of mRNA Vaccine in Lipid Nanoparticles

Shai Zilberzwige‐Tal, Aviad Levin, Assaf Ezra, Uri Elia, Gal Finkelstein‐Zuta, Topaz Kreiser, Lihi Gershon, Meir Goldsmith, Edo Kon, Yoav Navon, Ofer Cohen, Tuomas PJ Knowles, Dan Peer and Ehud Gazit

Small, 2025, 21
DOI: 10.1002/smll.202500114

Recent Advances in Microfluidic Technologies for the Construction of Artificial Cells

Siyuan Tan, Yongjian Ai, Xinchi Yin, Zhichao Xue, Xiang Fang, Qionglin Liang, Xiaoyun Gong and Xinhua Dai

Adv Funct Materials, 2023, 33
DOI: 10.1002/adfm.202305071