Intracellular evaluation of protein droplet-forming capability using self-assembling peptide tags

Takayuki Miki, Masahiro Hashimoto, Masatoshi Shimizu, Hiroki Takahashi and Hisakazu Mihara

Chem. Sci., 2025, 16, 14384
DOI: 10.1039/D5SC00871A

Effects of Hydrophobic Residues on the Intracellular Self-Assembly of De Novo Designed Peptide Tags and Their Orthogonality

Takayuki Miki, Keigo Kajiwara, Sae Nakayama, Masahiro Hashimoto and Hisakazu Mihara

ACS Synth. Biol., 2022, 11, 2144
DOI: 10.1021/acssynbio.2c00058

A General and Convenient Peptide Self‐Assembling Mechanism for Developing Supramolecular Versatile Nanomaterials Based on The Biosynthetic Hybrid Amyloid‐Resilin Protein

Junjun Wu, Lin Zhou, Hu Peng, Zhaojun Wang, Zhaoshi Wang, Jay D Keasling, Shike Liu, Guanghong Zhou, Shijie Ding, Qiong Wang, Xuejian Wang, Xinxiu Chen, Yifei Lang, Mo Xia, Xin Guan, Mingsheng Dong, Jingwen Zhou and Jian Chen

Advanced Materials, 2024, 36
DOI: 10.1002/adma.202304364

De novo designed YK peptides forming reversible amyloid for synthetic protein condensates in mammalian cells

Takayuki Miki, Masahiro Hashimoto, Hiroki Takahashi, Masatoshi Shimizu, Sae Nakayama, Tadaomi Furuta and Hisakazu Mihara

Nat Commun, 2024, 15
DOI: 10.1038/s41467-024-52708-5

Proximity labeling and identification of endogenous client proteins recruited to Y15‐based artificial granules tethering a bait protein

Masahiro Hashimoto, Takayuki Miki, Tatsuya Niwa and Hisakazu Mihara

Journal of Peptide Science, 2024, 30
DOI: 10.1002/psc.3536