Issue 12, 2007

Quantitative detection of protein expression in single cells using droplet microfluidics

Abstract

We demonstrate that single cells can be controllably compartmentalized within aqueous microdroplets; using such an approach we perform high-throughput screening by detecting the expression of a fluorescent protein in individual cells with simultaneous measurement of droplet size and cell occupancy.

Graphical abstract: Quantitative detection of protein expression in single cells using droplet microfluidics

Supplementary files

Article information

Article type
Communication
Submitted
19 Des 2006
Accepted
16 Jan 2007
First published
26 Jan 2007

Chem. Commun., 2007, 1218-1220

Quantitative detection of protein expression in single cells using droplet microfluidics

A. Huebner, M. Srisa-Art, D. Holt, C. Abell, F. Hollfelder, A. J. deMello and J. B. Edel, Chem. Commun., 2007, 1218 DOI: 10.1039/B618570C

To request permission to reproduce material from this article, please go to the Copyright Clearance Center request page.

If you are an author contributing to an RSC publication, you do not need to request permission provided correct acknowledgement is given.

If you are the author of this article, you do not need to request permission to reproduce figures and diagrams provided correct acknowledgement is given. If you want to reproduce the whole article in a third-party publication (excluding your thesis/dissertation for which permission is not required) please go to the Copyright Clearance Center request page.

Read more about how to correctly acknowledge RSC content.

Social activity

Spotlight

Advertisements