Issue 3, 2024

Split G-quadruplex based PfAgo sensing platform for nucleotide mutation discrimination and human genotyping

Abstract

A PfAgo-G4 sensing platform exploiting G4 as a signal reporter was proposed, validated, and optimized. By introducing two mismatches at the Link strand, a universal nucleotide design rule was established for accurate single nucleotide polymorphism discrimination with PfAgo-G4. The FUT2 gene was then successfully and accurately genotyped using human buccal swab samples.

Graphical abstract: Split G-quadruplex based PfAgo sensing platform for nucleotide mutation discrimination and human genotyping

Supplementary files

Article information

Article type
Communication
Submitted
04 Des 2023
Accepted
02 Jan 2024
First published
17 Jan 2024

Analyst, 2024,149, 707-711

Split G-quadruplex based PfAgo sensing platform for nucleotide mutation discrimination and human genotyping

Y. Zhang, B. Gong, Y. Lin, Y. Zhu, G. Su and Y. Yu, Analyst, 2024, 149, 707 DOI: 10.1039/D3AN02090H

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